新生大鼠海马齿状回神经干细胞的鉴定及褪黑激素对其分化的影响

目的 :探索新生大鼠海马齿状回神经干细胞分离培养及鉴定方法 ,并观察不同剂量褪黑激素对神经干细胞分化的影响。方法 :从新生大鼠海马齿状回分离得到神经干细胞 ,采用无血清培养技术进行培养 ,并采用 10、10 0 μmol/L褪黑激素诱导分化。应用细胞免疫化学方法进行神经干细胞巢蛋白 (nestin)、神经元特异性烯醇化酶 (neuronspecificenolase ,NSE)、胶质纤维酸蛋白 (glialfibrillaryacidicprotein ,GFAP)检测以进行细胞鉴定。结果 :鉴定表明从新生大鼠海马齿状回分离培养的细胞具有神经干细胞的特征 ,并可在体外增殖 ,经诱导可分化为神经元、星型胶质细胞和少突胶质细胞。褪黑激素能促进神经干细胞向神经元分化 ,但低浓度组与高浓度组的褪黑激素对神经元的分化并无显著性差异。结论 :从新生大鼠海马齿状回成功分离培养了神经干细胞 ,是研究细胞诱导分化的良好模型。褪黑激素能促进神经干细胞向神经元分化。

Identification of neural stem cells from dentate gyrus of hippocampus in newborn rats and influences of melatonin on differentiation of neural stem cells

Objective: To establish a method of isolation and culture of neural stem cells (NSCs) from dentate gyrus of hippocampus of neonatal rats.Methods: The cells isolated from dentate gyrus of neonatal rats were culutured in serum free medium.The cells were induced by melatonin and identified by immunocytochemistry. Results: The cells isolated from dentate gyrus of hippocampus of neonatal rats ,which had the characteristics of neural stem cells and were capable of proliferation in vitro,could be differentiated into neurons,astroglias and oligodendrosytes. The cells could be induced into neurons by melatonin. Conclusion: Neural stem cells are successfully isolated and cultured from dentate gyrus of hippocampus of neonatal rats. It is a favorable model for studies on inducedement of differentiation.Melatonin can indude neural stem cells into neurons.