中药丹参和骨碎补对大鼠正畸牙牙齿移动的比较研究

目的：研究中药丹参和骨碎补在大鼠正畸牙移动过程中对其牙周组织改建的作用。方法：选取72只SPF级Wistar雌性大鼠,随机分为丹参组、骨碎补组和对照组三组,每组24只,建立大鼠正畸牙齿移动实验模型,丹参组每日灌服6 g/kg丹参水煎剂,骨碎补组每日灌服6 g/kg骨碎补水煎剂,对照组每日灌服3 ml生理盐水,每隔7 d加力1次。三组动物于正畸加力7、14、21、28 d分批次处死,每次6只;剥离头颅骨,测量牙齿移动的距离及牙槽骨的密度,同时制作上颌第一磨牙区牙周组织切片,光学显微镜观察牙周组织改建情况,并进行统计学分析。结果：丹参组、骨碎补组牙齿移动距离均大于对照组,差异有统计学意义（P〈0.05）,丹参组和骨碎补组之间差异无统计学意义（P〉0.05）;光镜下观察三组的破骨细胞数量均呈现先增加后变平缓趋势,丹参组和骨碎补组较对照组增加更为显著（P〈0.05）,丹参组和骨碎补组之间差异无统计学意义（P〉0.05）;三组的牙槽骨密度都呈现降低趋势,丹参组和骨碎补组较对照组降低缓慢（P〈0.05）,丹参组和骨碎补组之间差异无统计学意义（P〉0.05）。结论：灌服丹参和骨碎补水煎液可促进大鼠牙周膜内破骨细胞生成并维持牙槽骨密度,有利于大鼠正畸牙齿移动。

A comparative study about the effects of salvia miltiorrhiza and drynaria on the orthodontic tooth movement

Objective：To observe the effects of salvia miltiorrhiza and drynaria on the construction of paradentium in orthodontic teeth movement.Methods：72 female 8-week-old SPF Wister rats were selected.They were randomly divided into three groups：the salvia miltiorrhiza group,the drynaria group and the control group.Then the animal model for orthodontic tooth movement was established.Every day,6 g/kg water apozem of salvia miltiorrhiza,6 g/kg water apozem of drynaria and 3 ml normal saline were drenched to the three groups respectively.Strengthened the force every 7 days.The rats were executed in batch on the 7th,14th,21st,28th days after orthodontic treatment.Peeled off the skull,distanced the tooth movement and measured the alveolar bone density.The tissue slices from the periodontium of the maxillary first molar were observed under optical microscope.Data was analyzed with PASW statistics 18.Results：Distances of the teeth movement in the salvia miltiorrhiza group and the drynaria group were greater than the control group（P0.05）,while no significant difference was found between the two experimental groups（P0.05）.The light microscope showed that the number of osteoclasts in all groups increased rapidly in the beginning,but stabilized later.The amount of the osteoclasts in the two experimental groups both increased more dramatically than the control group（P0.05）,whereas no significant difference were observed between themselves（P0.05）.Although the alvelor bone density presented to a downward trend in all groups,the two experimental groups declined slowly（P0.05）,and no difference was discovered between them（P0.05）.Conclusions：Gavage of salvia miltiorrhiza and drynaria decoction can promote the formation of osteoclast in the periodontal membrane of rats and maintain the alveolar bone density,which is conducive to orthodontic tooth movement in rats.