尼古丁受体3基因启动子多态与被动吸烟者发生肺癌的易感性研究

目的:探讨被动吸烟人群尼古丁受体3基因(CHRNA3)启动子区遗传变异与肺癌发病的关系.方法:用病例一对照的研究方法,收集250例被动吸烟原发性肺癌患者和250例性别相同、年龄相差士1岁被动吸烟正常对照,采用PCR-RFLP技术检测CHRNA3基因启动子单核苷酸多态位点rs6495309(T>C)的基因型,用SAS 9.13软件进行非条件Logistic回归校正混杂因素的影响,分析基因变异与肺癌发病的关联.结果:基因型频率分析均符合Hardy-Wein-berg平衡,P=0.109 6;CHRNA3rs6495309(T>C)住点变异在肺癌与对照中的分布差异有统计学意义,P=0.043 2;携带CC基因型个体发生肺癌的危险性较TT基因型个体增加81%(95%CI=1.07～3.06;P=0.019);rs6495309C变异基因型(rs6495309TC和rs6495309CC)患肺癌的危险性增加(adjusted OR=1.63;95%CI=1.06～2.50;P=0.025).发现被动吸烟来自于父母人群时该位点变异在肺癌与对照中分布差异有统计学意义,P=0.047 9.结论:CHRNA3基因启动子rs6495309(T>C)变异的被动吸烟人群肺癌发病危险性较高,且其危害性主要表现为被动吸烟来源于父母的人群.

Association between the genetic variant in CHRNA3 promoter and lung cancer risk in passive smoking population

OBJECTIVE: To investigate the association between the genetic variant in CHRNA3 promoter and lung cancer risk in passive smoking population.Methods: A case-control study of 250 lung cancer cases and 250 age-and sex frequency-matched cancer-free passive smoking controls were performed in a Chinese population to investigate the risk of lung cancer for common polymorphism rs6495309(T>C).CHRNA3 genotype was determined by PCR-RFLP analysis and SAS 9.13 was used to analysis the genetic variant and lung cancer ris...