| Livin靶向siRNA对大肠癌细胞凋亡的诱导作用 |
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| 引用本文： | 蔡明,王国斌,陶凯雄,韩高雄,蔡昌学.Livin靶向siRNA对大肠癌细胞凋亡的诱导作用[J].中华肿瘤防治杂志,2010,17(12). |
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| 作者姓名： | 蔡明 王国斌 陶凯雄 韩高雄 蔡昌学 |
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| 作者单位： | 1. 华中科技大学同济医学院附属协和医院普外科,湖北,武汉,430022
2. 华中科技大学同济医学院病原生物系微生物学教研室,湖北,武汉,430030 |
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| 基金项目： | 国家863高技术研究发展计划资助项目，湖北省科技攻关计划资助项目 |
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| 摘 要： | 目的:构建Livin靶向小干扰RNA(siRNA)重组表达载体,观察其诱导大肠癌细胞的凋亡效应.方法:构建Livin靶向siRNA重组表达栽体并转染结肠癌细胞,RT-PCR和蛋白质印迹法检测Livin的表达,MTT法检测siRNA对细胞增殖的抑制作用,流式细胞仪检测细胞的凋亡效应.结果:经酶切鉴定和测序结果证实,Livin靶向sirNA重组表达载体构建成功,它对大肠癌细胞Livin mRNA和蛋白的表达抑制率分别为30.18%和28.88%,P<0.05,肿瘤细胞的生长受到明显抑制,细胞凋亡率为(13.36±1.45)%,P<0.05.结论:Livin靶向siR-NA重组表达载体构建成功,能有效抑制Livin基因表达并能显著诱导肿瘤细胞凋亡.
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| 关 键 词： | 结直肠肿瘤 RNA 干扰 基因治疗 |
Apoptosis induction effect of siRNA recombinant expression vector targeting Livin gene in human colon cancer cells |
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| CAI Ming,WANG Guo-bin,TAO Kai-xiong,HAN Gao-xiong,CAI Chang-xue.Apoptosis induction effect of siRNA recombinant expression vector targeting Livin gene in human colon cancer cells[J].Chinese Journal of Cancer Prevention and Treatment,2010,17(12). |
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| Authors: | CAI Ming WANG Guo-bin TAO Kai-xiong HAN Gao-xiong CAI Chang-xue |
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| Institution: | CAI Ming1,WANG Guo-bin1,TAO Kai-xiong1,HAN Gao-xiong1,CAI Chang-xue21.Department of General Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,P.R.China2.Department of Pathogenic Biology,School of Basic Medical Sciences,Wuhan 430030,P.R.China |
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| Abstract: | OBJECTIVE:To construct siRNA recombinant expression vector targeting Livin gene and observe the apoptosis induction effect of it in human colon cancer cells.METHODS:siRNA recombinant expression vector targeting Livin gene was constructed and transfected into human colon cancer cells.The effect of siRNA recombinant expression vector was detected by RT-PCR,Western blot,MTT reduction assay and flow cytometry.RESULTS:It was confirmed by restriction endonuclease and sequence analysis that siRNA recombinant expre... |
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| Keywords: | Livin |
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