In vivo evaluation of the inhibitory capacity of human plasma on exogenous surfactant function |
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Authors: | Prof Dr B Lachmann E P Eijking K L So D Gommers |
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Institution: | (1) Department of Anesthesiology, Room Ee 2393, Erasmus University, P.O. Box 1738, NL-3000 DR Rotterdam, The Netherlands |
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Abstract: | Objective The adult respiratory distress syndrome (ARDS) and neonatal respiratory distress syndrome (RDS) are characterized by high
permeability pulmonary edema which contains plasma-derived proteins inhibiting pulmonary surfactant function. Currently, discussion
continues as to what dose of surfactant is required for treatment of these syndromes.
Design The purpose of this study was to investigate the amount of exogenous surfactant needed to overcome the inhibitory components
in human plasma. Male adult rats suffering from respiratory failure due to surfactant depletion after whole-lung lavage received
human plasma (4 ml/kg body weight) mixed with surfactant at different concentrations, intratracheally. Rats receiving surfactant
only at different concentrations served as controls. Blood gas analysis was performed.
Measurements and results It was demonstrated that plasma (4 ml/kg≈273 mg, plasma proteins/kg) mixed with surfactant at 300 mg/kg was able to increase
and maintain PaO2 at normal values. Plasma mixed with surfactant at 100 mg/kg, after initial restoration of blood gases, showed deterioration
of PaO2 values. Plasma mixed with surfactant at a dose of 50 mg/kg did not improve PaO2 whereas surfactant at 50 mg/kg, without plasma, restored blood gases to pre-lavage values.
Conclusion It is concluded that approximately 1 mg surfactant phospholipids is required to overcome the inhibitory effect of approximately
1 mg plasma proteins. For clinical practice this means that an excess of surfactant should be given, or repeatedly be substituted
(“titrated”) at low concentrations, until blood gases improve.
This work was financially supported by the Dutch Foundation for Medical Research |
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Keywords: | Animal model Blood gases Respiratory failure Exogenous surfactant therapy Surfactant inhibition Plasma proteins |
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