Anticancer activity of total flavonoids isolated from Xianhe Yanling Recipe (仙鹤延龄方) |
| |
作者姓名: | 刘培勋 高境 陈毓杰 龙伟 沈秀 唐卫生 |
| |
作者单位: | Institute of Radiation MedicineChinese Academy of Medical Sciences,Peking Union Medical College,Tianjin Key Laboratory of Molecular Nuclear Medicine |
| |
摘 要: | Objective:To investigate the anticancer activity of the total flavonoids isolated from a herbal formula,Xianhe Yanling Recipe(仙鹤延龄方),a recipe commonly used in cancer patients in China.Methods: The in vitro anticancer activity of the total flavonoids was determined using the 3-(4,5-dimethylthiazole-2-yl)-2,5- diphenyltetrazolium bromide(MTT) assay on three cancer cell lines:MCF-7(a human breast adenocarcinoma cell line),HepG-2(a human hepatocellular carcinoma cell line) and ES-2(a human ovarian cancer cell line). The in vivo anticancer effect of the total flavonoids was assessed in a mouse tumor model bearing H22-induced hepatocellular carcinoma,and cisplatin was used as a positive control.Results:The total flavonoids exerted a powerful inhibitory effect on the three cell lines,with 50%inhibiting concentrations(IC50) of 24.948,31.569 and 6.923μg/mL,respectively.In vivo studies showed that the total flavonoids had dose-dependent inhibitory effects on hepatocellular carcinoma in mice.Conclusion:The total flavonoids from Xianhe Yanling Recipe have potential anticancer activity,and further researches and development are warranted.
|
关 键 词: | anticancer flavonoids Xianhe Yanling Recipe 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide assay in vivo |
收稿时间: | 2011-01-20 |
本文献已被 CNKI 维普 PubMed SpringerLink 等数据库收录! |
| 点击此处可从《中国结合医学杂志》浏览原始摘要信息 |
|
点击此处可从《中国结合医学杂志》下载免费的PDF全文 |
|