SATB1在鼻咽癌中的表达及意义

目的研究富含AT序列的特异结合蛋白1（special AT rich sequence binding protein 1,SATB1）在鼻咽癌（nasopharyngeal carcinoma,NPC）中的表达及意义。方法运用免疫组化法检测72例原发性NPC和30例鼻咽粘膜慢性炎症组织（chronic nasopharyngitis tissue,CNT）中SATB1蛋白的表达,并对NPC的临床病理参数进行统计分析。运用荧光定量PCR的方法检测高成瘤高转移鼻咽癌细胞株（SUNE-1亚株-5-8F）和只成瘤不转移鼻咽癌细胞株（SUNE-1亚株-6-10B）中SATB1的mRNA表达水平。结果 NPC和CNT中SATB1蛋白的阳性表达率分别为52.8%（38/72）和13.3%（4/30）,与CNT比较,NPC中SATB1表达上调,差异有统计学意义（P〈0.05）。NPC伴有淋巴结转移和无淋巴结转移SATB1阳性表达率分别为64.3%（27/42）和36.7%（11/30）,有远处转移和无远处转移的阳性表达率分别为88.9%（8/9）和47.6%（30/63）,两者比较,差异均有统计学意义（P〈0.05）。NPC原发灶中SATB1高表达与患者性别、年龄、T分级和临床分期无关（P〉0.05）,与N分级及M分级有关（P〈0.05）。此外,SATB1mRNA在5-8F细胞的表达量为6-10B细胞的4.09倍。结论 SATB1表达上调可能参与NPC的侵袭和转移。

Expression of SATB1 in nasopharyngeal carcinoma and its significance

Objective To investigate the expression and significance of SATB1 in nasopharyngeal carcinoma(NPC).Methods Immunohistochemical staining method was used to detect the expression of SATB1 protein in 72 NPC samples and 30 samples of chronic nasopharyngitis tissue(CNT),and the correlations between SATB1 expression and clinicopathological parameters were evaluated.The expression of SATB1 mRNA was also investigated in 5-8F and 6-10B of human nasopharyngeal carcinoma cell lines by quantitative real-time PCR(qRT-PC...