急性百草枯中毒大鼠肺组织MDA、T-SOD、GSH水平及三七皂苷的干预作用

目的观察百草枯(PQ)急性中毒大鼠血浆、肺组织匀浆中丙二醛(MDA)、总超氧化物歧化酶(T-SOD)和谷胱甘肽还原酶(GSH)水平,探讨三七皂苷(PNS)对PQ中毒大鼠的抗氧化治疗作用。方法将54只SD大鼠随机分为3组,即对照组(Control组)、PQ组、PQ+PNS组。PQ组、PQ+PNS组采用1次性灌胃构建SD大鼠中毒模型,后者同时给予PNS预防性治疗,采用化学比色法检测肺匀浆中MDA、T-SOD和GSH的含量。结果PQ组和PQ+PNS组血浆和肺匀浆中MDS高于Control组,T-SOD、GSH低于Control组(P<0.01)。PQ组与PQ+PNS组比较,PQ+PNS组中MDA含量在血浆和肺匀浆中均较PQ组降低(P<0.01);PQ+PNS组血浆中T-SOD高于PQ组(P<0.01);肺匀浆中T-SOD高于PQ组(P<0.05);PQ+PNS组血浆和肺匀浆中GSH含量高于PQ组(P<0.05)。结论三七皂苷可抑制由PQ诱导的过氧化反应,对PQ中毒具有抗氧化治疗作用。

The MDA,T-SOD and GSH levels in lung tissue of rat with acute paraquat poisoning and intervention of notoginsenoside

Objective To determine the malondialdehyde(MDA),superoxide dismutase(T-SOD) and glutathione reductase(GSH) levels in rat plasma and lung homogenates of acute paraquat(PQ) poisoning,and to explore Panax notoginseng saponins on the role of antioxidant treatment to PQ poisoning rats.Methods 54 SD rats were randomly divided into three groups: Control group,PQ group and PQ + PNS group.PQ group and PQ + PNS group with disposable gavages to establish the SD rat poisoning model,the latter at the same time give PNS preventive treatment,the chemical colorimetric assay in lung homogenates of malondialdehyde(MDA),total superoxide dismutase(T-SOD) and glutathione reductase(GSH) content.Results The MDS in the plasma and lung homogenates was higher in the PQ group and PQ + PNS group than that in control group,the T-SOD and GSH was lower than that in control group(P<0.01).Compared with PQ group,PQ + PNS group contents of MDA in plasma and lung homogenates was lower(P<0.01);The plasma T-SOD was higher in PQ + PNS group than that in PQ group(P0.01);T-SOD in the lung homogenate was higher than that in PQ group(P<0.05);GSH content was higher than that in PQ group(P0.05) in plasma and lung homogenate of the PQ + PNS group.Conclusion Notoginsenoside can inhibit PQ-induced peroxidation,have antioxidant treatment to PQ poisoning.