应用ATPase组化法及TEM法对皮肤癣菌病患者表皮郎格罕细胞的观察

本文应用ATPase组化方法首次对30例皮肤癣菌病患者皮损表皮及70例对照者相应部位表皮作了郎格罕细胞(LC)观察,并对其中3例患者及1例对照者表皮LC作了透射电镜(TEM)观察。结果发现患者表皮LC形态改变、数量减少或消失及细胞超微结构受损;且上述改变程度随病变范围扩大及病程延长而加重。本文首次提出LC与皮肤癣菌病的免疫病理改变有密切关系,可能皮肤癣菌的入侵使表皮LC受损,从而干扰了T淋巴细胞诱导的机体对病原体的清除功能。本文还讨论了两种方法的注意点。

The Investigations on Epidermal Langerhans Cell of the Dermatophytosis Patients Using ATPase Histochemical Method and TEM

The investigations of Langerhans cell (LC) in involved epidermides of 30 dermatop-hytosis patients and in corresponding positions of 70 controls were made by using ATP-ase staining, of which 3 patients and 1 control were observed by using TEM. The results showed that the numbers of LC of the patients were diminished or disappeared, the shapes were changed, and the ultrastructure were damaged. These changs were related to the course and the forms of the eruptions of the patients. The close correlation of LC with the immunopathogenesis in derma tophytosis was preliminarily unveiled. Since the invading dermatophytes impairs the LC, it is possible that the discharging function to the patient induced by T-cell is interfered. In addition, the important aspects of these two methods are discussed.