| PCR-ELISA用于HFRS病人早期诊断的研究 |
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| 引用本文： | 韦三华,白雪帆,潘蕾,李光玉,陈红梅. PCR-ELISA用于HFRS病人早期诊断的研究[J]. 中国人兽共患病杂志, 2002, 18(1): 76-78 |
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| 作者姓名： | 韦三华 白雪帆 潘蕾 李光玉 陈红梅 |
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| 作者单位： | 第四军医大学唐都医院感染病防治中心,第四军医大学唐都医院感染病防治中心,第四军医大学唐都医院感染病防治中心,第四军医大学唐都医院感染病防治中心,第四军医大学唐都医院感染病防治中心 西安710038,西安710038,西安710038,西安710038,西安710038 |
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| 摘 要： | 目的 建立检测HFRS患者血清标本中HV基因的PCR -ELISA方法。方法 设计并合成互补于HVS基因片段的标记引物 ,对 98例HFRS患者血清进行RT -nestedPCR扩增 ,并用酶免方法分析扩增产物。结果 98例患者不同病日采集的血清标本的RT -nestedPCR平均检出率仅为 6 0 .2 % ,而PCR -ELISA平均检出率为 80 .6 %。结论 PCR -ELISA用于早期HFRS患者的HV基因检测是一种理想的检测方法
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| 关 键 词： | 肾综合征出血热 多聚酶链反应 PCR-ELISA |
| 文章编号： | 1002-2694(2002)01-0076-03 |
| 收稿时间： | 2002-01-20 |
| 修稿时间： | 2001-01-10 |
Early diagnosis for patients with HFRS by PCR-ELISA |
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| WEI Sanhua,BAI Xuefan,PAN Lei,LI Guangyu,CHENG Hongmei. Early diagnosis for patients with HFRS by PCR-ELISA[J]. Chinese Journal of Zoonoses, 2002, 18(1): 76-78 |
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| Authors: | WEI Sanhua BAI Xuefan PAN Lei LI Guangyu CHENG Hongmei |
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| Abstract: | Aim To establish a rapid and sensitive method for detecting Hantavirus (HV) genes in patient sera of HFRS by PCR ELISA. Method:The primers labeled by digoxin and biotin and complemented to S segment of HV were synthesized and RT nested PCR was conducted for detecting HV genes in patient sera of HFRS.The amplified products were analyzed by ELISA.Result:The 98 patients serm of HFRS patients were tested by RT nested PCR and the positive rate was 60/2%;by PCR ELISA the positive rate was 80.61%.Conclusion:PCR-ELISA is a more sensitive method for early diagnosis of HFRS. |
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| Keywords: | Hemorrhagic fever with renal syndrome Polymerase chain reaction PCR ELISA |
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