Mapping of the herpes simplex virus DNA sequences present in herpes simplex virus type-1 thymidine kinase-transformed cells |
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Authors: | Jeffrey M. Leiden Niza Frenkel Dennis Sabourin Richard L. Davidson |
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Affiliation: | (1) Department of Biology, The University of Chicago, 60637 Chicago, Illinois;(2) Department of Microbiology and Molecular Genetics, Harvard Medical School, USA;(3) Division of Human Genetics, Children's Hospital Medical Center, 02115 Boston, Massachusetts |
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Abstract: | Analyses of the herpes simplex virus (HSV) DNA sequences which are present in three HSV thymidine kinase-transformed (HSVtk+)mouse cell lines have revealed that these cells contain relatively large and variable portions of the viral genome. Two of these cell lines do not contain the viral DNA sequences known to encode the early viral genes normally responsible for regulating tkgene expression during lytic HSV infections. This finding suggests that cell-associated viral tkgene expression may be regulated by cellular rather than viral control mechanisms. In addition, we have compared the viral DNA sequences present in one unstable HSVtk+cell line to those present in tk–revenant and tk+rerevertant cell lines sequentially derived from it. Our results have shown that within the limits of sensitivity of our mapping approach, these three related cell lines contain the same set of viral DNA sequences. Thus, gross changes in viral DNA content do not appear to be responsible for the different tk phenotypes of these cells. |
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