三氧化二砷对体外培养A375黑色素瘤细胞的生长抑制作用及机制初步研究

目的 为了解三氧化二砷(As2O3)对体外培养的A375黑色素瘤细胞的生长及谷胱甘肽过氧化物酶活力和血管生长因子(VEGF)含量的影响.方法 采用四甲基偶氮唑蓝(MTT)还原法检测1.50、3.00、6.00、12.00、24.00 μ mol/L As2O3与分别与A375黑色素瘤细胞作用24h、48h、72h后对黑素瘤细胞的抑制作用;测定作用24h的A375黑色素瘤细胞所含的谷胱甘肽过氧化物酶活性及VEGF含量.结果 相同作用时间内,各浓度的As2O3对A375瘤细胞的生长均有明显的抑制作用,As2O3的浓度越高,对培养的A375瘤细胞的增殖抑制作用越强,经过统计学独立样本的t检验分析,差别有统计学意义;各浓度As2O3与A375黑色素瘤细胞作用24h后,瘤细胞的谷胱甘肽过氧化物酶活力均有明显的降低,在As2O3浓度为6.0μmol/L时,A375瘤细胞的酶活性相对最高,低于此浓度或高于此浓度时,酶活力呈递减的趋势;VEGF含量均有增加,As2O3,浓度为3.0μmol/L时达到峰值,低于此浓度或高于此浓度时呈递减的趋势.经过统计学独立样本的t检验分析,差别均有统计学意义.结论 As2O3对A375瘤细胞的生长有明显的抑制作用,并呈现浓度依赖性;作用24h,As2O3显著降低A375瘤细胞谷胱甘肽过氧化物酶活力,并增加VEGF的含量.

Arsenic trioxide affect the A375 melanoma cell growth and glutathione peroxidase activity in vitro

Objective To understand the effect of arsenic trioxide (As2O3) on cultured A375 melanoma cell growth and glutathione peroxidase activity and VEGF content.Methods By use of methyl thia-zolyl tetrazolium (MTT) reduction assay at 1.50, 3.00, 6.00, 12.00, 24.00 μ mol/L As2O3 reduced A375 melanoma cells growth in 24h, 48h, 72h respectively.Determining the glutathione peroxidase activity and VEGF content of the A375 melanoma cells co-cultured with As2O3 for 24 hours, respectively.Results At the same time point, the growth of A375 tumor cells with the various concentrations of As2O3were significantly inhibited, the higher concentration of As2O3 in cultured A375 tumor cell, the stronger proliferation inhibitory effect was observed.For 24 hours, the glutathione peroxidase activity of the A375 melanoma cells with As2O3 was significantly lower, and with 6.0 μ mol/L of As2O3 A375 tumor cells were in the highest relative activity, below or higher this concentration, the enzyme activity trend decreased.At the first 24 hours, the VEGF was increased to the peak with As2O3 at3.0 μ mol/L, while the VEGF was down trend with either higher or low-erAs2O3. The both difference was statistically significant with independent samples T-test.Conclusions As2O3 inhibits the growth of A375 tumor cells significantly, and shows a dose-dependent manner. As2O3 reduce the A375 tumor cell glutathione peroxidase activity and increase the VEGF content in the first 24 hours repetitively.