SLE患者外周血白细胞B7-H1分子的表达

目的 探讨B7-H1分子在系统性红斑狼疮(SLE)患者外周血白细胞的表达.方法 心用荧光抗体染色技术,经流式细胞仪检测30例SLE患者和20例正常人对照者外周血白细胞表面B7-H1的自然表达水平,同时收集SLE患者的实验室检查指标,并用SLE疾病活动指数(SLEDAI)来判定患者疾病活动程度.结果 活动期SLE组单核细胞、淋巴细胞和粒细胞上B7-H1表达水平均明显高于正常人对照组(P<0.01);SLE患者单核细胞和淋巴细胞B7-H1表达水平与SLEDAI呈正相关(r₁=0.42,P₁<0.05;r₂=0.52,P₂<0.05);抗dsDNA抗体阳性患者组淋巴细胞表面B7-H1分子数较抗dsDNA抗体阴性组明显增高(t=3.20,P<0.01).治疗后SLE患者外周血单核细胞、淋巴细胞和粒细胞B7-H1表达均明显下降,t值分别为10.00、6.75和4.33,P<0.01.结论 活动期SLE患者外周血白细胞B7-H1表达增加,且与SLEDAI成正相关,B7-H1可能与SLE的发病机制有关.

B7-H1 expression on peripheral blood mononuclear cells from patients with systemic lupus erythematosus

Objective To investigate the expression of B7-H1 on peripheral blood mononuclear cells(PBMCs) from patients with systemic lupus erythematosus(SLE).Methods Immunofluorescent staining and flow cytometry assay were used to measure the expression level of B7-H1 on PBMCs from 30 SLE patients and 20 healthy controls.Results of laboratory examination of patients were collected.SLE disease activity index(SLEDAI) was used to evaluate the disease severity.Results The expression levels of B7-HI on monocytes,lymphocytes and granulocytes was markedly higher in active SLE group than those in control group(all P<0.01),respectively.The expression of B7-H1 on monocytes and lymphocytes was positively correlated with SLEDAI of patients(r₁=0.42,P₁<0.05; r₂=0.52,P₂<0.05),respectively.On the other hand,B7-H1 expression on lymphocytes was significantly higher in patients with positive anti-dsDNA antibody than that in parients with negative anti-dsDNA antibody(t=3.20,P<0.01).After three weeks' treatment with glucocorticoid,the level of B7-Hl was significantly decreased on the monocytes,lymphocytes and granulocytes of patients in comparison with that before treatment(t=10.00,6.75,4.33,respectively,all P < 0.01).Conclusions The expression of B7-H1 on PBMCs increased in active SLE,and there was positive correlation between B7-Hl expression and SLEDAI,which suggests that B7-Hl may be relevant to the pathogenesis of SLE.