| 大黄酚对LoVo细胞AQP2表达的调节效应 |
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| 引用本文： | 刘青,李锋,任秦有,王新,王文,王长海,杜永平.大黄酚对LoVo细胞AQP2表达的调节效应[J].第四军医大学学报,2009,30(9):849-852. |
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| 作者姓名： | 刘青 李锋 任秦有 王新 王文 王长海 杜永平 |
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| 作者单位： | 刘青,李锋,王文,王长海,杜永平,LIU Qing,LI Feng,WANG Wen,WANG Chang-Hai,DU Yong-Ping(西京医院中医科,全军中医内科中心,陕西,西安,710033);任秦有,REN Qin-You(唐都医院中医科,陕西,西安,710038);王新,WANG Xin(西京医院消化内科,全军消化病研究所,陕西,西安,710033)
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| 摘 要： | 目的：探讨大黄酚对体外培养LoVo细胞AQP2表达的调节效应.方法：体外培养LoVo细胞,随机分为对照组,大黄酚10,20,40mg/L不同浓度组.间接免疫荧光定性LoVo细胞AQP2表达,WesternBlot、半定量RT-PCR检测药物处理24h后AQP2蛋白及mRNA表达.体外培养LoVo细胞后,随机又将细胞分为对照组,大黄酚40mg/L组,PKA激动剂8-Bromo-cAMP10mg/L组,大黄酚40mg/L＋PKA激动剂8-Bro-mo-cAMP10mg/L组.非放射性法检测药物处理24h后LoVo细胞PKA的活性水平.结果：AQP2表达于LoVo细胞膜,药物处理24h后,大黄酚20,40mg/L组AQP2蛋白分别为（0.64±0.08）,（0.46±0.09）显著低于对照组（0.83±0.05）,（P〈0.01）;AQP2mRNA分别为（0.50±0.12）,（0.39±0.09）,显著低于对照组（0.73±0.08）,（P〈0.01）.40mg/L大黄酚组PKA活性（0.54±0.08）显著低于对照组（0.78±0.10）,（P〈0.05）.结论：大黄酚可抑制LoVo细胞AQP2基因转录与翻译.大黄酚对AQP2表达的调节效应可能与大黄泻下作用有关,其可能是通过PKA信号通路调节AQP2的表达.
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| 关 键 词： | 大黄酚 LoVo细胞系 水通道蛋白2 药理作用 |
Regulating effect of aquaporin2 expression by chrysophanol on intestinal epithelial cell line LoVo |
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| LIU Qing,LI Feng,REN Qin-You,WANG Xin,WANG Wen,WANG Chang-Hai,DU Yong-PingPLA Centre of Traditional Chinese Medicine.Regulating effect of aquaporin2 expression by chrysophanol on intestinal epithelial cell line LoVo[J].Journal of the Fourth Military Medical University,2009,30(9):849-852. |
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| Authors: | LIU Qing LI Feng REN Qin-You WANG Xin WANG Wen WANG Chang-Hai DU Yong-PingPLA Centre of Traditional Chinese Medicine |
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| Institution: | LIU Qing1,LI Feng1,REN Qin-You2,WANG Xin3,WANG Wen1,WANG Chang-Hai1,DU Yong-Ping11PLA Centre of Traditional Chinese Medicine,Department of Traditional Chinese Medicine,3PLA Institute of Gastroenterology,Xijing Hospital,Fourth Military Medical University,Xi'an 710033,China,2Department of Traditional Chinese Medicine,Tangdu Hospital,Xi'an 710038 |
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| Abstract: | AIM:To investigate the effect of chrysophanol in regulating AQP2 in LoVo cells cultured with RPMI-1640 medium containing chrysophanol. METHODS: LoVo cells cultured with RPMI-1640 medium in vitro were randomly divided into 4 groups: control group, chrysophanol treatment groups(10, 20 and 40 mg/L, respectively). The location of AQP2 was decided by indirect immunofluorescene and the expression levels of protein and mRNA of AQP2 after a 24 h treatment were decided by Western Blot and semiquantive RT-PCR. LoVo c... |
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| Keywords: | chrysophanol LoVo cell line aquaporin2/AQP2 pharmacologic action |
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