结缔组织病患者咽部定植的白念珠菌毒力测定

目的了解结缔组织病患者咽部定植的白念珠菌芽管生成长度和分泌型天冬氨酸蛋白酶活性。方法采用病例对照方法对病例组30株和健康组4株白念珠菌芽管生成长度和分泌型天冬氨酸蛋白酶活性进行测定。结果病例组菌株芽管长度为(8.425±1.513)μm,显著高于健康组的(6.495±0.375)μm(P<0.05);病例组分泌型酸性蛋白酶活性Pa值为0.490±0.053,低于健康组的0.610±0.056(P<0.01)。白念珠菌生成的芽管长度与其分泌的酸性蛋白酶活性Pa值呈负性相关(r=-0.874,P<0.01)。结论与健康人相比,结缔组织病患者咽部定植的白念珠菌毒力较强,有更强的潜在致病力。

Investigation on the Virulence of Candida Albicans Isolated from Throat in Patients with CTDs

Objective To investigate the germ tube growth and secreted aspartic proteinase activity so as to evaluate the virulence level of candida albicans strains isolated from throats in patients with connective tissue diseases(CTDs).Methods A caes-control study was used to compare the length of germ tube and the activity of secreted aspartic proteinase between the patient group and the healthy group.Results The length of germ tube was 8.425±1.513μm in patient group and 6.495±0.375μm in healthy group. The former was significantly longer than the latter(P<0.05). The activity of secreted aspartic proteinase (Value Pa)was 0.490±0.053 in patient group and 0.610±0.056 in healthy group. The difference between two groups was significant,too(P<0.05). It also showed that the length of germ tube was negatively correlated with the activity of secreted aspartic proteinase(r=-0.874,P<0.01).Conclusion The virulence and the potential pathogenicity of candida albicans isolated from throat in patients with CTDs were both higher than the those in healthy ones.