Burst production of superoxide anion in human endothelial cells by lysophosphatidylcholine |
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Authors: | Kugiyama K Sugiyama S Ogata N Oka H Doi H Ota Y Yasue H |
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Affiliation: | Division of Cardiology, Kumamoto University School of Medicine, Japan. kiyo@gpo.kumamoto-u.ac.jp |
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Abstract: | This study examined whether lysophosphatidylcholine (lysoPC), an atherogenic lipid, may stimulate production of O2*-, in cultured human endothelial cells. Production of O2*- was detected by bis-N-methylacridinium nitrate (lucigenin)-elicited chemiluminescence. LysoPC was found to induce burst production of O2*-, peaked at 2-4 min after the stimulation, in intact endothelial cells. LysoPC also stimulated NADH-dependent production of O2*- in particulate fraction of the cells, and the action of lysoPC was inhibited by diphenyliodonium. The results suggested that lysoPC stimulated production of O2*- partly through membrane-associated NADH-dependent O2*- production systems. |
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