A human monoclonal antibody against HLA-A25 |
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| Authors: | S. Kosinski G. B. Ferrara S. Y. Yang U. Haummerling |
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| Affiliation: | Memorial Sloan-Kettering Cancer Center, New York, N.Y., U.S.A.;Instituto Nazionale per la Ricerca suo Cancro, Genova, Italy |
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| Abstract: | We are reporting the production and characterization of a human monoclonal antibody recognizing antigen HLA-25. The antibody was developed by a line transformed in vitro by the Epstein-Barr virus. The immune B lymphocytes for transformation were generated by planned immunization of a volunteer with repeated doses of allogenic peripheral blood lymphocytes of one donor over the course of 7 years. The antibody showed correlation with A25 antigen on a panel of 244 individuals tested by microcytotoxicity. The antibody showed neither cytotoxic reactivity nor CYNAP phenomenon with antigens of HLA-10 CREG. |
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