应用巢式PCR检测原发性肝癌患者血清中HCV—RNA

为了探讨原发性肝癌的发生是否与丙型肝炎病毒有关,应用巢式PCR技术和ELISA检测40例原发性肝癌患者及50例除外肝病患者血清中HCV—RNA及抗HCV抗体和乙肝二对半。结果:原发性肝癌组中,乙肝二对半阳性37例(92.5％),抗HCV阳性8例(20％),HCV—RNA阳性13例(32.5％),HCV总感染率为35.0％(14例);对照组分别为16例(40.0％),2例(4.0％)及3例(6.0％)。在3例HBV—M阴性PHC患者中,HCV—RNA阳性2例。结论:在我国PHC的发生不仅与乙肝病毒感染有关,还与丙型肝炎病毒有关。

DETECTION OF HCV-RNA IN SERUM FROM PATIENTS WITH PRIMARY HEPATOCELLULAR CARCINOMA USING NESTED PCR

In order to further investigate the correlation between primary hepatocellular carcinoma (PHC) and the hepatitis C virus,nested PGR and ELISA assays were used to detect the presence of HCV-RNA, anti-HCV, and markers of the hepatits B virus. Samples taken from 40 patients with PHC and 50 individuals without liver disease were analysed. In the PHCgroup,HCV-RNA,and markers of the hepatitis B virus were respectively detected in 13 cases(32. 5%),8 cases (20%),and 37 cases (92. 5%). Total HCV infection in PHC patients was found in 14 of the patients (35%),in the control group,the number of cases with detectable HCV-RNA,anti HCV,and markers of the hepatitis B virus were significantly lower,6. 0% , 4. 0%and 40. 0%. HCV-RNA was detected in two of the three HBV-M(-)PHC patients. We have observed that there is not only a relationship between PHC and HBV, but also that a correlation between PHC and HCV exists.