疏肝清脂颗粒延缓或消退动脉粥样硬化斑块机制的实验研究

目的探讨疏肝清脂颗粒降低血脂、抑制动脉粥样硬化的机理。方法（1）选取新西兰大耳白兔60只，通过喂饲高脂饲料，造成高脂血症动物模型，分为6组（动脉粥样硬化模型对照组、疏肝清脂低剂量组、疏肝清脂高剂量组、中药对照组、西药对照组及正常对照组）。（2）通过生化、放免检测，从血管活性物质方面探讨疏肝清脂颗粒对内皮功能的影响。（3）采用酶联免疫吸附法（ELISA）测定氧化修饰低密度脂蛋白（oxidized low-density lipoprotein，ox-LDL）和肿瘤坏死因子154（CD40／CD40L）；应用RT-PCR方法检测基质金属蛋白酶（matrix metalloproteninase-2，MMP-2，9）。结果疏肝清脂低、高剂量组，中药对照、西药对照及正常对照组OX-LDL水平明显低于模型对照组，差异有显著性（P〈0．01）；疏肝清脂低、高剂量组及西药组MMP-2及MMP-9蛋白含量明显低于模型对照组，差异有显著性（P〈0．01）。疏肝清脂低、高剂量组及西药组TIMP-2明显高于模型对照组，差异有显著性（P〈0．01）。结论疏肝清脂颗粒通过防止炎性细胞黏附，抑制平滑肌细胞增殖和迁移，减少细胞外基质产生，进而稳定斑块，最终使动脉粥样硬化发生和发展受到抑制并缓解。

Experimental Study on Mechanism of Shugan Qingzhi Granule for Stabilizing or Reducing the Plaque of Atherosclerosis

Objective To explore the mechanism of Shugan Qingzhi Granule (SGQZG) for reducing blood lipids level and anti-atherosclerosis. Methods Hyperlipidemia rabbit model was established by high-fat diet. Sixty New Zealand rabbits were randomly divided into 6 groups, the model group (A), the high and low dose SGQZG group (B and C), the Chinese medicine control group (D), the Western medicine control group (E), and the normal group (F), 10 in each group. The effect of SGQZG on vascular endothelial functions was determined by biochemistry and radioimmunoassay; activity of oxidized low-density lipoprotein (ox-LDL) and tumor necrosis factors CD40/CD40L analyzed by ELISA; and matrix metalloproteinase-2 and -9 (MMP-2 and MMP-9) detected using RT-PCR. Results As compared with the model group (Group A) the ox-LDL level was lower in Group B-F, MMP-2 and MMP-9 were lower and TIMP-2 was higher in Group B, C and E, all showed statistical significance (P<0.01). Conclusion SGQZG may alleviate and regress the genesis and development of atherosclerosis through preventing the adhesion of inflammatory cells, inhibiting proliferation and migration of smooth muscle cells, and reducing the production of extracellular matrix to stabilize the plaque.