大鼠栓塞肺组织的比较蛋白质组学研究

目的研究大鼠血栓栓塞肺组织和正常肺组织的蛋白差异表达。方法采用血栓颈静脉注入法制备大鼠急性肺栓塞模型，采用双向电泳技术（2-DE）找出差异蛋白，用MALDI-TOF技术和生物信息学技术鉴定差异蛋白，并对部分差异表达蛋白采用Westernblot技术作进一步验证。结果肺组织蛋白的2-DE胶银染可分离出2800多个点，考染可达到2400个点以上。经图像分析得到的46个差异表达蛋白中有32个蛋白得到鉴定。对部分差异蛋白采用Westernblot方法在蛋白水平的验证结果与2-DE结果基本相符。结论采用比较蛋白质组学的方法可以发现大量栓塞肺组织的差异表达蛋白，这些差异表达蛋白将为研究肺栓塞发病的分子机制和病理生理学研究提供重要的线索和依据。

Comparative proteomic study of acute pulmonary embolism in rats

Objective To study differential expression of proteins in rat acute pulmonary embolism(PE)model.Methods The acute PE rat model was constructed by injecting 3-4 emboli into the left jugular vein.The differentially expressed proteins in the lung tissues were separated and identified at different time points using two-dimensional electrophoresis(2-DE)and matrix assisted laser desorbtion/ionization time-of-flight mass spectrometer(MALDI-TOF-MS).Part of the identified proteins were validated by western blot analysis.Results There were approximately 2 800 protein spots in every 2-DE gels of lung tissue samples visualized by silver staining,and 2 400 protein spots using Coomassie Brilliant Blue R-250.Among 46 differentially displayed protein spots,32 found their corresponding protein candidates in the database.The results of western blot analysis were accordance with that of 2-DE.Conclusion The differential expression of these proteins may play an important role in different stages of the rat PE model,and provide clues for uncovering the pathophysiologic molecular mechanisms involved in PE.