| 胎鼠肺发育中PI3K-AKT信号传导通路效应物表达 |
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| 引用本文： | 王志辉,刘淑娟,王君,王玉洪,王虹. 胎鼠肺发育中PI3K-AKT信号传导通路效应物表达[J]. 青岛大学医学院学报, 2008, 44(6) |
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| 作者姓名： | 王志辉 刘淑娟 王君 王玉洪 王虹 |
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| 作者单位： | 青岛市第八人民医院心内科,山东,青岛,266100 |
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| 摘 要： | 目的 探讨1-磷脂酰肌醇3-激酶-丝氨酸-苏氨酸蛋白激酶B(PI3K-AKT)信号传导通路效应物在胎鼠肺发育过程中的表达.方法 运用Western蛋白印迹方法 检测不同胎儿期鼠肺上皮细胞中AKT、磷酸化AKT (pAKT)及相关蛋白的表达.运用免疫组织化学方法 检测不同胎儿期鼠肺上皮细胞中pAKT的表达水平.结果 Western 蛋白印迹法显示,AKT在不同胎儿期肺上皮细胞中的蛋白表达无明显差异;第12天胎鼠肺pAKT高度表达,第14天开始减少;第12天胎鼠肺cyclinD1高度表达,第14天开始减少;第12天胎鼠肺SPC低度表达,第14天开始逐渐增加.免疫组织化学法显示,第14天胎鼠肺上皮细胞中pAKT高度表达,第18天胎鼠肺上皮细胞中pAKT无明显表达.结论 PI3K-AKT信号传导通路通过许多生物过程在胎鼠肺发育中起重要作用.
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| 关 键 词： | 丝氨酸-苏氨酸蛋白激酶B 肺发育 小鼠,近交ICR |
THE EXPRESSION OF PI3K-AKT SIGNAL PATHWAY PROTEINS DURING DEVELOPMENT OF FETAL MOUSE LUNG |
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| WANG ZHI-HUI,LIU SHU-JUAN,WANG JUN,et al. THE EXPRESSION OF PI3K-AKT SIGNAL PATHWAY PROTEINS DURING DEVELOPMENT OF FETAL MOUSE LUNG[J]. Acta Academiae Medicinae Qingdao Universitatis, 2008, 44(6) |
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| Authors: | WANG ZHI-HUI LIU SHU-JUAN WANG JUN et al |
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| Affiliation: | WANG ZHI-HUI,LIU SHU-JUAN,WANG JUN,et al(Department of Endocrinology,No.8 People's Hospital of Qingdao,Qingdao 266100,China) |
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| Abstract: | Objective To study the effect of PI3K-AKT signal pathway in fetal mouse lung development. Methods The expression of AKT,phosphorylated AKT(pAKT) and the downstream proteins were examined with Western blotting and immunohistochemistry in the epithelium of fetal mouse lung at different time points. Results Western blotting showed that the expression of AKT was not significantly changed in embryonic lungs during development,while phosphorylated AKT(pAKT) was highly expressed on embryonic day 12(E12) and starte... |
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| Keywords: | Serine/threonine protein kinase B Lung development Mice inbred ICR |
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