儿童哮喘与白三烯受体1及C4合成酶基因多态性的关系

目的探讨白三烯受体1(CYSLTR1)和白三烯C4合成酶(LTC4S)基因多态性与上海儿童哮喘及其严重程度之间的关系。方法 2006年9月至2009年8月本院儿科住院及门诊儿童哮喘患者72例,男39例,女33例,平均年龄(5.1 1.6)岁。根据哮喘发作程度分为间歇和轻度发作组44例、中重度发作组28例。健康对照组成人95例,男50例,女45例。应用连接酶检测反应(LDR)技术,对CYSLTR1的927T/C位点和LTC4S的444A/C位点基因多态性进行分析。结果①哮喘组和对照组927T/C位点基因型、等位基因频率比较(χ2=0.178,0.04,P(0.05)、444A/C位点基因型、等位基因频率比较(χ2=1.14,1.065,P(0.05)差异均无统计学意义。②927T/C和444A/C位点基因型频率在哮喘各组中的分布比较(χ2=0.927,1.138,P(0.05)差异均无统计学意义。结论在上海哮喘儿童中,CYSLTR1的927T/C位点和LTC4S的444A/C位点基因多态性可能不是其发病和严重程度的主要基因危险因素。

Cysteinyl leukotrienes receptor 1 and leukotriene C4 synthetase genetic polymorphism in asthmatic children

Objective To observe the relationship between cysteinyl leukotrienes receptor 1（CYSLTR1）and leukotriene C4 synthetase（LTC4S） genetic polymorphism and severity of asthma in asthmatic children of Shanghai area.Methods Seventy-two asthmatic patients hospitalized or in outpatient clinic were included from September 2006 to August 2009.Among them there were 39 males and 33 females with mean age（5.1±1.6） years.According to the severity of asthma the patients were divided into mild intermittent and persistent group（44 patients）,moderate severe group（28 patients）.Ninety-five healthy adults（50 males and 45 females） had also been recruited.The genetic polymorphisms were assessed with ligase chain reaction（LCR） technique.Results ①There was no significant difference in the genotype and allele frequency of 927T/C（χ^20.178,0.04,P﹥0.05）and 444A/C（χ^21.14,1.065,P﹥0.05）between asthma group and control group.②There was no significant difference in the genotype frequency of 927T/C and 444A/C in asthma groups（χ^20.927,1.138,P﹥0.05）.Conclusion In Shanghai asthmatic children,CYSLTR1 927T/C and LTC4S 444A/C genetic polymorphisms might not be associated with the pathogenesis of asthma and severity.