长苞凹舌兰提取物抗亚急性衰老小鼠氧化损伤的作用

目的观察长苞凹舌兰提取物(Coeloglossum.viride var.bracteatum extract,CE)对D-半乳糖(D-Gal)和NaNO2致衰老小鼠氧化损伤的作用.方法雌性NIH小鼠适应性饲养1周后,将动物随机分为:正常对照组,衰老模型组,吡拉西坦(阳性对照)组300mg/(kg·d)],CE低、中、高剂量组2.5、5、10 mg/(kg·d)]6组,每组10只.衰老模型组,吡拉西坦组和CE低、中、高剂量组小鼠接受腹腔注射D-Gal 120 mg/(kg·d)(生理盐水配制)和NaNO2 90 mg/(kg·d)(双蒸水配制),连续60 d,正常对照组小鼠腹腔注射等量的生理盐水溶液;从第47天开始,吡拉西坦组和CE低、中、高剂量组小鼠分别灌胃相应剂量的吡拉西坦300mg/(kg·d)]或CE2.5、5、10 mg/(kg·d)],正常对照组和衰老模型组小鼠灌胃等量的生理盐水溶液.14d后用水迷宫方法检测小鼠的学习记忆能力;生物化学法检测脑中丙二醛(MDA)水平和超氧化物歧化酶(SOD)、单胺氧化酶A(MAO-A)、单胺氧化酶B(MAO-B)、钠钾ATP酶、钙镁ATP酶活力.结果衰老模型组小鼠每天游出迷宫所用的时间(潜伏期)较正常对照组明显延长(P＜0.01,P＜0.05),错误次数明显增多(P＜0.05);小鼠脑中SOD、钠钾ATP酶、钙镁ATP酶的活力下降(P＜0.01,P＜0.05),MDA水平、MAO-A、MAO-B活力升高(P＜0.01).灌胃给予吡拉西坦300mg/(kg·d)]和CE25、5、10mg/(kg·d)]可以改善衰老模型小鼠的上述变化.结论CE可改善D-Gal和NaNO2致衰老小鼠的学习记忆障碍,具有促智和延缓衰老的作用,此作用可能部分由于CE具有抗氧化、改善能量代谢障碍和抑制单胺类递质代谢失调的作用.

Effect of Coeloglossum. viride var. bracteatum extract on oxidation injury in sub-acute senescent model mice

OBJECTIVE: To study the anti-aging effects of Coeloglossum. viride (L) Hartm. var. bracteatum (Willd) Richter extract (CE) on senescent model mice induced by D-galactose and sodium nitrite. METHODS: After one week of accommodation, 60 female NIH mice were divided into six groups with 10 mice in each group: normal control group, aging model group, Piracetam (positive control) group 300 mg/ (kg x d)], and CE reatment groups 2.5, 5, and 10 mg/ (kg x d)]. Mice in aging model group, Piracetam group, and CE treatment group were consecutively intraperitoneally injected with D-galactose 120 mg/ (kg x d)] and sodium nitrite 90 mg/ (kg x d)] for 60 days. From day 47, mice in Piracetam group and CE treatment group were po given Piracetam 300 mg/ (kg x d) or CE 2.5, 5, and 10 mg/ (kg x d). Mice in normal control group and aging model group were po given saline. The drug administration lasted for 14 days. Water maze test was performed to evaluate the learning and memory function in the mice. The activity of superoxide dismutase (SOD), the content of malondialdehyde (MDA), and the activities of adenosinetriphosphatase (ATPase), and monoamine-oxidase (MAO) in brain tissue were measured. RESULTS: The latencies in water maze test in aging model group mice were significantly longer than in normal control group (P < 0.01, P < 0.05), and the number of errors increased (P < 0.05). In aging model group mice, the activities of SOD, Na+K(+)-ATPase, and Ca2+Mg(2+)-ATPase decreased (P < 0.01, P < 0.05), while the content of MDA and the activities of MAO-A and MAO-B increased (P < 0.01). Piracetam 300 mg/ (kg x d), po] and CE 2.5, 5, 10 mg/ (kg x d), po] ameliorated the above changes in aging model mice. CONCLUSION: CE may improve the memory dysfunction induced by consecutive injection of D-galactose and sodium nitrite,and has nootropic and antiaging effects.