基于UPLC特征图谱和含量测定的不同产地筋骨草质量评价

目的 建立筋骨草超高效液相色谱法（UPLC）特征图谱和其中哈巴苷、乙酰哈巴苷的含量测定方法。方法 采用Waters BEH C₁₈色谱柱（150 mm×2.1 mm，1.7 μm），以乙腈-甲醇（4∶1）为流动相A、0.2%磷酸水溶液为流动相B，梯度洗脱，流速为0.30 mL·min^–1，柱温为30 ℃，检测波长为202 nm，进样量为2 μL，建立筋骨草特征图谱，测定其中哈巴苷、乙酰哈巴苷的含量。结果 17批筋骨草特征图谱有10个共有峰，指认了其中4个成分分别为哈巴苷、乙酰哈巴苷、木犀草素-7-二葡萄糖苷酸、芹菜素-7-二葡萄糖苷酸；17批筋骨草中哈巴苷质量分数为0.33%~0.95%，乙酰哈巴苷质量分数为0.47%~1.93%。结论 建立的UPLC特征图谱和哈巴苷、乙酰哈巴苷含量测定方法可为筋骨草质量评价提供参考。

Quality Evaluation of Ajuga decumbens from Different Producing Areas Based on UPLC Characteristic Chromatogram and Content Determination

Objective To establish the characteristic chromatogram of Ajuga decumbens Thunb. by ultra-high performance liquid chromatography (UPLC) and method for determining the content of harpagoside and acetyl harpagoside.Methods Waters BEH C₁₈ (150 mm×2.1 mm, 1.7 μm) column was used. The mobile phase A was acetonitrile-methanol (4:1), and the mobile phase B was 0.2% phosphoric acid aqueous solution for gradient elution. The flow rate was 0.30 mL·min^–1, and the column temperature was 30 ℃. The detection wavelength was 202 nm, and the injection volume was 2 μL. The characteristic chromatogram of A. decumbens was established to determine the content of harpagoside and acetyl harpagoside.Results A total of 10 common fingerprint peaks were identified in the characteristic chromatograms of 17 batches of A. decumbens, among which four components were identified as harpagoside, acetyl harpagoside, luteonin-7-diglucosidic acid, and apigenin-7-diglucosidic acid. The mass fraction of harpagoside in the 17 batches of A. decumbens. was in the range of 0.33%-0.95%, while that of acetyl harpagoside was 0.47%-1.93%.Conclusion The established UPLC characteristic chromatogram and the method for determining the content of harpagoside and acetyl harpagoside can provide a basis for the quality evaluation of A. decumbens.