NMR-based metabolomics with enhanced sensitivity |
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| Authors: | Kousik Chandra,Samah Al-Harthi,Sujeesh Sukumaran,Fatimah Almulhim,Abdul-Hamid Emwas,Hanudatta S. Atreya,Ł ukasz Jaremko,Mariusz Jaremko |
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| Affiliation: | Biological and Environmental Science and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), 23955-6900 Thuwal Saudi Arabia.; NMR Research Centre, Indian Institute of Science, Bangalore 560012 India ; Core Laboratories, King Abdullah University of Science and Technology (KAUST), 23955-6900 Thuwal Saudi Arabia |
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| Abstract: | NMR-based metabolomics, which emerged along with mass spectrometry techniques, is the preferred method for studying metabolites in medical research and food industries. However, NMR techniques suffer from inherently low sensitivity, regardless of their superior reproducibility. To overcome this, we made two beneficial modifications: we detuned the probe to reach a position called “Spin Noise Tuning Optimum” (SNTO), and we replaced the conventional cylindrical 5 mm NMR tube with an electric field component-optimized shaped tube. We found that concerted use of both modifications can increase the sensitivity (signal to noise ratio per unit volume) and detection of metabolites and decrease the measurement time by order of magnitude. In this study, we demonstrate and discuss the achieved signal enhancement of metabolites on model non-human (bovine serum, amino acid standard mixture) and human urine samples.We combined Spin Noise Tuning Optimum (SNTO) and electric field component-optimized shaped tube to boost sensitivity for NMR-based metabolomics. |
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