A signal-on fluorescent aptasensor based on gold nanoparticles for kanamycin detection |
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| Authors: | Yimeng Sun Tong Qi Yan Jin Lijuan Liang Jianlong Zhao |
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| Affiliation: | State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050 China.; Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing 100049 China ; College of Sciences, Shanghai Institute of Technology, Shanghai 201418 China |
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| Abstract: | This study describes the development, verification and practical application of an aptasensor for the fluorometric detection of kanamycin. Using the nucleic acid aptamer with FAM fluorescent group as the conjugate, using gold nanoparticles as the fluorescence dynamic quenching source, a fluorescence sensor was fabricated through the signal-on method for the micro-detection of kanamycin. The nucleic acid chimera is connected to the fluorophore, and the gold nanoparticles are used as the fluorescence dynamic quenching source under actual conditions. The detection limit of kanamycin is 0.1 pM, and the detection range is 0.1 pM to 0.1 μM. This biosensor works satisfactorily in complex samples with no impurities, which gives this method an obvious advantage over other analytical methods. In addition, the mechanism of action between gold nanoparticles/FAM–aptamer/kanamycin is discussed and studied in depth here. It provides a more thorough analysis and more application possibilities for fluorescence-aptamer biosensing.This study describes the development, verification and practical application of an aptasensor for the fluorometric detection of kanamycin. |
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