Mapping of murine YACs containing the genesCea2 andCea4 after B1-PCR amplification and FISH-analysis期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Mapping of murine YACs containing the genesCea2 andCea4 after B1-PCR amplification and FISH-analysis

Authors:	G. Rettenberger W. Zimmermann C. Klett U. Zechner H. Hameister

Affiliation:	(1) Abteilung Medizinische Genetik, Universität Ulm, Albert-Einstein-Allee 11, 89069 Ulm, Germany;(2) Institut für Immunbiologie, Universität Freiburg, Stefan-Meier Str. 8, 79104 Freiburg, Germany;(3) Abteilung Medizinische Genetik, Universität Ulm, Germany;(4) Present address: the Institut für Nutztierwissenschaften, Eidgenössische Technische Hochschule ETH-Z, Tannenstrae 1, CH-8092 Zürich, Switzerland

Abstract:	PCR with primers specific for the murine B1 consensus sequence allows amplification of DNA from murine sources. We have used B1-PCR for amplifying yeast artificial chromosome (YAC) DNA which can be used to localize single YACs by fluorescencein situ hybridization. The genes for the pregnancy-specific glycoproteins Cea2 and Cea4, both belonging to the large carcinoembryonic antigen gene family, were localized by chromosomalin situ suppression hybridization of three YAC clones to murine chromosome 7A2-A3. This was facilitated by the use of the mouse lymphoma cell line WMP/WMP which contains nine pairs of Robertsonian fusion chromosomes.

Keywords:	B1-primers carcinoembryonic antigen FISH gene mapping mouse PCR pregnancy-specific glycoprotein
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