Cytomorphological,cytogenetic, and molecular biological characterization of four new human renal carcinoma cell lines of the clear cell type |
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Authors: | C D Gerharz U Ramp N Marx H E Gabbert J Olert R Moll S Störkel |
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Institution: | (1) Department of Pathology, University Hospital of Düsseldorf, Moorenstrasse 5, D-40225 Düsseldorf 1, Germany;(2) Department of Pediatric Pathology, University Hospital of Mainz, Langenbeckstrasse 1, D-55101 Mainz, Germany;(3) Department of Pathology, University Hospital of Mainz, Mainz, Germany |
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Abstract: | Four new permanent cell lines (RCC-A, -B,-C, and -D) derived from different human renal cell carcinomas of the clear cell type were established in tissue culture. The cell lines displayed characteristic differences in cell size and shape, which allowed individual identification by phase contrast microscopy. Ultrastructurally, the cell lines exhibited varying amounts of cytoplasmatic glycogen and lipid. Immunohistochemistry revealed co-expression of vimentin and cytokeratin in all cell lines. The mean population doubling time ranged from 27 h (RCC-A) to 104 h (RCC-D). RCC-B and -C cells produced slowly growing tumours after heterotransplantation into nude mice, whereas RCC-A and RCC-D cells were non-tumorigenic. The modal chromosome number was either near-diploid (RCC-A, -B, and -C) or near triploid (RCC-D). Clonal abnormalities affecting the short arm of chromosome 3 were seen in all cell lines. Northern blot analysis revealed no expression of the proto-on-cogenes c-fos, c-ros, and c-mos, whereas c-Ki-ras expression was observed in all cell lines. Expression of c-myc was observed in RCC-A, RCC-B, and RCC-D cells, whereas c-raf expression could be detected in RCC-B and RCC-D. Tumour suppressor gene p53 mRNA was observed in the cell line RCC-D. |
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Keywords: | Human renal cell carcinoma Cell line Proto-oncogene Tumour suppressor gene p53 |
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