| HPLC法测定非诺贝特软胶囊中维生素E的含量 |
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| 引用本文： | 戚燕,王春艳,吴松.HPLC法测定非诺贝特软胶囊中维生素E的含量[J].中国药品标准,2014,15(5):341-343. |
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| 作者姓名： | 戚燕 王春艳 吴松 |
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| 作者单位： | 中国医学科学院 北京协和医学院药物研究所活性物质的发现与适药化研究北京市重点实验室,北京100050 |
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| 基金项目： | 国家“重大新药创制科技重大专项基金项目”（编号：2013ZX09402103） |
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| 摘 要： | 目的：建立非诺贝特软胶囊中维生素E含量测定方法。方法：采用HPLC法，色谱柱为Thermo BDS Hypersil C184.6mm×150 mm，5μm），流动相为甲醇-水（98∶2），流量1.0mL· min^-1，检测波长为284nm，柱温40℃。结果：维生素E在0.5～102μg· mL^-1浓度范围内线性关系良好，平均回收率为99.3％（RSD＝0.36％）。结论：所建方法专属性好，准确，重复性好，可为非诺贝特软胶囊中有效检测抗氧剂维生素E的含量提供参考。
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| 关 键 词： | 非诺贝特软胶囊 维生素E 高效液相色谱法 |
| 收稿时间： | 2014/6/11 0:00:00 |
| 修稿时间： | 2014/8/26 0:00:00 |
Determination of Vitamin E in Fenofibrate Soft Capsules by HPLC |
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| Authors: | Qi Yan Wang ChunYan and Wu Song |
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| Institution: | (Institute of Materia Medica, Chinese Academy of Medical Sciences ＆ Peking Union Medical College, Beijing City Key Laboratory of Active Substance Discovery and Druggability Evaluation, Beijing 100050) |
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| Abstract: | Objective：To establish an HPLC method for the determination of Vitamin E in fenofibrate soft capsules.Methods：Vitamin E was extracted by ethanol with ultrasonic extraction.Thermo BDS Hypersil C18 column（4.6 mm ×150 mm,5 μm） was used with methanol-water （98∶2） as the mobile phase at the flow rate of 1.0 mL· min^-1.The detection wavelength was 284 nm and the column temperature was 40 ℃.Results：There was a good linearity in the range of 0.5-102 μg· mL^-1 , the average recovery was 99.3%with RSD =0.36%.Conclusion：The method is specific, accurate and repeatable and can be used for the determination of Vitamin E in fenofibrate soft capsules. |
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| Keywords: | fenofibrate soft capsules Vitamin E HPLC |
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