不同性别人群的蛋白质Ｎ 糖基结构谱型比较

摘要：目的　对不同性别人群的血浆Ｎ 糖基结构谱型进行分析比较，为基础人群Ｎ 糖基结构的分布提供
科学依据。方法　采用整群抽样，以２００９年４～７月在北京某社区调查的２１２名居民为研究对象，基于高
效液相色谱法进行血浆糖基组检测。不同性别的糖基含量比较采用两独立样本狋检验或非参检验，犘＜０．０５
为差异有统计学意义。结果　２１２名研究对象的平均年龄为（３７．７８±１７．８９） 岁，性别比为０．８８。半乳糖
基化修饰水平，男性双触角非半乳糖聚糖水平略高（犣＝２．３３，犘＝０．０２）， 女性单半乳糖聚糖水平较高
（狋＝－２．０６，犘＝０．０４）。岩藻糖基化分析显示，女性核心岩藻糖化聚糖水平略高（狋＝ －２．１６，犘＝０．０３）。
唾液酸糖基化水平中，女性单唾液酸糖基化水平较高（狋＝－２．４３，犘＝０．０２），男性二唾液酸糖基化水平较
高（狋＝２．１１，犘＝０．０４）。结论　血浆Ｎ 糖基结构水平可能存在性别决定性。
关键词：蛋白质Ｎ 糖基化修饰；性别；高效液相色谱
中图分类号：Ｒ１９５　　文献标识码：Ａ　　文章编号：１００９ ６６３９ （２０１４）０６ ０５６６ ０５

Comparison of structure profiles of protein N-glycosylation between two genders

Objective To analyze and compare the structure profiles of plasma N-glycosylation between two genders in order to provide scientific basis for the distribution of N-glycosylation. Methods A cluster sampling method was used to randomly select 212 residents from a community in Beijing.Blood samples were collected and plasma N-glycans were identified by high performance liquid chromatography（HPLC）. ResultsThe average age of subjects was 37.78±17.89 years with the sex ratio of 0.88.The levels of galactosylation,fucosylation and Sialic glycosylation of N-glycans were all significantly different between male and female subjects（P〈0.05）,with more monogalactosylated glycans,core-fucosylated glycans and monosialo in women,and more biantennary non-galactosylated glycans and disialo in men. Conclusions The plasma N-glycome profile is gender dependent,therefore,it is very important to consider this difference when the glycome markers are to be developed.