血管能抑素基因转移抑制视网膜新生血管的实验研究

目的 观察血管能抑素真核表达质粒(pCMV-HA)对小鼠视网膜新生血管RNV形成的抑制作用.方法 将鼠龄为7 d的56只C57BL/6J新生小鼠随机分为正常对照组、氧诱导视网膜病变(OIR)模型组、治疗组和空载体组,每组14只.后3组小鼠置于(75±2)%浓度的氧环境中饲养5 d后,回到正常空气环境中建立氧诱导的RNV动物模型.治疗组小鼠在出生后第12天出氧箱时行玻璃体腔注射血管能抑素pCMV-HA,空载体组注射等量空质粒.出生后第17天行伊凡思蓝(Evans blue)灌注血管造影视网膜铺片观察血管变化.石蜡切片行苏木精-伊红染色,光学显微镜下观察并计数突破视网膜内界膜的血管内皮细胞核数.结果 视网膜铺片结果显示,治疗组较OIR模型组和空载体组视网膜血管分布均匀,新生血管和无灌注区显著减少.治疗组突破内界膜的内皮细胞核数与OIR模型组及空载体组比较,差异有统计学意义(F=39.006,P<0.001).结论 血管能抑素pCMV-HA对氧诱导的RNV有显著的抑制作用.

Inhibitory effects of gene transfer of eanstatin on retinal neovascularization

Objective To observe the inhibitory effects of gene transfer of canstatin on retinal neovascularization in mice. Methods Fifty-six 7-day-old C57BL/6J mice were randomly divided into control group, oxygen-induced retinopathy (OIR) group, empty vector group and treated group, 14 mices in each group. Except for the control group, the mice in the other groups were exposed to (75±2)% oxygen for 5 days and then back to the normal air to establish the model of OIR. On postnatal 12 day, the treated group was received intravitreal injection of canstatin pCMV-HA, while the empty vector group was received the same volume of empty plasmid. The changes of retinal vessels were observed by Evans blue angiography on postnatal 17 day. With parafin section which stained by hematoxylin and eosin, then the number of endotheliocyte nuclei breaking throuhgh the internal limiting membrane(ILM) was observed and counted by optical microscope. Results Retinal blood vessels distributed regularly in treated group compared with OIR group and empty vector group. The differences of the number of endotheliocyte nuclei breaking throuhgh ILM in treated group was significant compared with the other two groups(F= 39. 006, P< 0. 001).Conclusion The canstatin pCMV-HA can effectively inhibit the retinal neovascularization in OIR.