体外不同张应力对人牙周膜成纤维细胞增殖活性的影响

目的 观察不同张应力对人牙周膜成纤维细胞(HPDLF)增殖活性的影响，进一步了解HPDLF的应力-生物学效应及其在正畸治疗中可能的生长增殖规律。方法 利用自行设计的膜式动态张应力-体外细胞培养研究系统对分离培养的HPDLF进行对照组(0kPa)、静张应力组(5kPa)、动态张应力组1(5kPa-0kPa-5kPa)、动态张应力组2(5kPa-2．5kPa-5kPa)不同水平及频动范围张应力加载，每组再分16h、24h和32h3个观察时间点，以^3H-TdR掺入法检测其S期细胞增殖活性。结果 静张应力对HPDLF具有明显的促增殖作用，有使其增殖高峰提前的趋向;动态张应力对HPDLF的增殖活性有一定的抑制作用。结论 不同张应力对HPDLF的增殖活性的影响明显不同，静张应力作用具有明显的促增殖作用，而动态张应力有一定的抑制作用。

Effect of Different Tension Stress on the Proliferation Activity of Human Peridontal Ligament Fibroblast in vitro

Objective The aim of this study was to examine the proliferation activity of human periodontal ligament fibroblasts (HPDLF) under different tension stress in vitro so as to learn the stress biological effect and growth regularity in orthodontic treatment of HPDLF. Methods HPDLF were harvested and subjected to the cellular mechanical research system, and the levels of proliferation activity under static and dynamic tension stress in different frequency and magnitude were detected by use of 3H TdR at 16 h, 24 h and 32 h. Results It was found that the proliferation activity of HPDLF obviously increased in the static tension stress group (5 kPa), whereas the proliferation activity of HPDLF appeared to decrease in the dynamic tension stress groups (5 kPa 2.5 kPa 5 kPa; 5 kPa 0 5 kPa). Conclusion Different tension stress patterns have different effects on the proliferation of HPDLF. Static tension stress can obviously enhance the proliferation of HPDLF.