IL-6对受照荷瘤小鼠正常组织的辐射防护效应

目的　以荷瘤小鼠为观察对象 ,研究白细胞介素 6 (Interleukin - 6 ,IL - 6 )对受照射荷瘤小鼠正常组织的辐射防护作用。方法　本实验用黄嘌呤氧化酶法测定受全身照射的荷瘤小鼠肝、肾、脑、肺组织中的总超氧化物歧化酶 (T -SOD)含量 ;用硫代巴比妥酸钠 (TBA)荧光法测定以上组织中的脂质过氧化物 (LPO)产物丙二醛 (MDA)的含量 ;双底物偶联动力学法测定以上组织中的谷光苷肽过氧化物酶 (GSH -PX)含量。结果　对于接受全身照射的荷瘤小鼠IL - 6可提高肝、脑组织中的抗氧化酶T -SOD、GSH -PX活性 (P <0 .0 1,P <0 .0 5 ) ,降低肝、脑组织中MDA含量 (P <0 .0 5 )。结论　IL - 6对受全身照射的荷瘤小鼠可肝、脑组织有辐射防护作用。

Radioprotective effects of IL-6 on normal tissues of irradiated tumor-bearing mice

Objective To study the radioprotective effects of Interleukin-6(IL-6) during radiotherapy,in this paper we observed tumor-bearing mice and discussed radioprotective effects of IL-6 on normal tissues of irradiated tumor-bearing mice.Methods The contents of Total Superoxide Dismutase in liver,kidney,brain and lung of irradiated tumor bearing mice were determined by the method of xanthineoxidase. The contents of MDA were determined by TBA fluorescence and the contents of glutathione peroxidase(GSH-Px) were determined by kinetics. Results IL-6 showed significant radioprotective effects on the irradiated brain and liver tissue,including accelerating activities of T-SOD, (P<0.01,P<0.05) and reducing contents of MDA(P<0.05). Conclusion IL-6 probably had radioprotective effects on the brain and liver tumor bearing mice after TBI.