慢乙肝患者外周血中Th17细胞趋化因子受体的表达水平

目的 检测慢性乙型病毒性肝炎（chronic hepatitis B,CHB）患者外周血中Th17细胞表面趋化因子受体CCR4、CCR6、CXCR3的表达,分析CCR4、CCR6、CXCR3与丙氨酸转氨酶（ALT）、总胆红素（TBil）及HBV DNA载量的相关性.方法 流式细胞仪检测30例CHB患者（CHB组）及15名健康人（对照组）外周血中Th17细胞CCR4、CCR6、CXCR3的表达,与ALT、TBil、HBV DNA载量进行相关性分析.结果 CHB组CD4＋ Th17细胞CCR4、CCR6、CXCR3的表达水平高于对照组,其差异有统计学意义（P＜0.05）.CHB组CD8＋ Th17细胞CCR4、CCR6的表达水平高于对照组,其差异有统计学意义（P＜0.05）.CCR4、CCR6表达水平与ALT、HBV DNA载量呈正相关（P＜0.05）,与TBil水平无相关性（P＞0.05）.结论 CHB患者外周血中Th17细胞表面CCR4、CCR6表达增高,与炎症程度相关,可能涉及Th17细胞引起的肝损伤.

Expression of chemokine receptors on Th17 cell in peripheral blood of patients with chronic hepatitis B

Objective To investigate the expression of chemokine receptors CCR4,CCR6,CXCR3 on Th17 cell,and analyze the correlation between chemokine receptors and serum biochemical parameters of liver function test and hepatitis B virus （HBV） DNA load in peripheral blood of chronic hepatitis B （CHB） patients.Methods Thirty patients with CHB （CHB group） and 15 healthy persons （control group） were enrolled in the study.CCR4,CCR6,CXCR3 expression levels on Th17 cell were assayed by flow cytometry.The correlation between chemokine receptors and alanine aminotransferase （ALT）,total bilirubin （TBil）,and HBV DNA load were analyzed using Pearson＇ s correlation analysis,respectively.Results CCR4,CCR6,CXCR3 expression levels on CD4 ＋ Th17 cell in CHB group were higher than that of control group.Moreover,the difference between them was statistically significant （P 〈 0.05）.CCR4,CCR6 expression levels on CD8 ＋ Th17 cell in CHB group were higher than that of control group （P 〈 0.05）.Although CXCR3 expression level on CD8 ＋ Th17 cell in CHB group was higher than that of control group,there was no significant difference between them （P 〉 0.05）.CCR4,CCR6 expression levels were positively correlated with serum ALT,HBV DNA load,respectively （P 〈 0.05）,but were not correlated with TBil （P 〉 0.05）.Conclusion Expression levels of chemokine receptor on Th17 cell were increased in patients with CHB and were positively associated with degree of liver inflammation.Therefore,CCR4,CCR6 expression on Th17 cell might be involved in liver injury resulted from Th17 cell.