| mPer2对小鼠B16黑色素瘤细胞增殖作用的影响 |
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| 引用本文： | 陈兢,李华琦,李浩,惠旭辉.mPer2对小鼠B16黑色素瘤细胞增殖作用的影响[J].四川生理科学杂志,2006,28(4):163-164. |
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| 作者姓名： | 陈兢 李华琦 李浩 惠旭辉 |
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| 作者单位： | 1. 四川大学华西医院,神经外科,610041
2. 内江市第一人民医院,内分泌科 |
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| 摘 要： | 目的:研究mPer2对小鼠黑色素瘤细胞B16增殖作用的影响。方法:将构建好的mPer2全长表达质粒(pcDNA3.1-mPer2)转染入小鼠黑色素瘤细胞B16中,并以pcDNA3.1空质粒转染为对照,通过平板克隆形成实验和MTT法检测两组细胞的增殖情况。结果:平板克隆形成实验和MTT法检测结果均显示Period2转染组小鼠黑色素瘤细胞B16较空质粒对照组明显降低。结论:mPer2的高表达能显著性的抑制小鼠黑色素瘤细胞B16的生长。
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| 关 键 词： | mPer2 细胞增殖 B16细胞 |
| 收稿时间： | 2006-08-21 |
| 修稿时间： | 2006年8月21日 |
The function of mper2 on cell proliferation of B16 mouse melanoma cell |
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| Chen Jing,Li Huaqi,Li Hao,Hui Xuhui.The function of mper2 on cell proliferation of B16 mouse melanoma cell[J].Sichuan Journal of Physiological Sciences,2006,28(4):163-164. |
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| Authors: | Chen Jing Li Huaqi Li Hao Hui Xuhui |
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| Abstract: | Objective:To study the function of mPer2 on cell proliferation of B16 mouse melanoma cell. Methods: pcDNA 3.1-mPer2 plasmids, pcDNA 3.1 plasmids and PBS were transfected into mouse melanoma sell B16. Colony formation of each group was test with methylene blue and growth-inhibitory effect of each group was detected by MTT assay. Results: Colony formation test and MTT assay showed that the cell proliferation of pcDNA 3.1-mPer2 group was more depressed than pcDNA 3.1 group and PBS group. Conclusion:The up-regulation of mPer2 expression in B16 mouse melanoma cell can remarkably depress the cell proliferation of B16. |
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| Keywords: | mPer2 Cell proliferation B16 cell line |
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