血中阿托品的高效液相色谱检测及灌流清除效率的实验研究

目的研究血中阿托品的高效液相色谱快速检测方法及血液灌流对硫酸阿托品的定量清除效率。方法模拟临床血液灌流装置,对含有硫酸阿托品的血样进行灌流吸附,用高效液相色谱法紫外检测器检测硫酸阿托品的残留量。结果低、中、高三种浓度的加标回收率为107.0%～110.7%,相对标准偏差为0.95%～7.8%。灌流前硫酸阿托品浓度为74.51μg/ml;在灌流1h、2h和3h后,血中残留硫酸阿托品浓度为：0.5g组分别是16.00μg/ml、3.85μg/ml、1.51μg/ml;1.0g组分别是6.45μg/ml、0.81μg/ml、0.21μg/ml;1.5g组分别是4.50μg/ml、0.43μg/ml、0.32μg/ml。结论该法能够做到快速准确地检测血中硫酸阿托品;包膜活性炭血液灌流同样对硫酸阿托品有较强的清除作用,临床灌流过程中要严格监测血中阿托品浓度,适时补充阿托品。

Determination of blood atropine by HPLC and experimental study on the clearance of atropine by hemoperfusion

Objective To establish the rapid determination of blood atropine and to study the clearance rate of blood atropine by hemoperfusion. Methods We performed perfusion and adsorption for healthy sheep blood mixed with atropine through an extracorporeal closed circulating perfusion apparatus loaded with membrane-coated activated charcoal, similar to that for clinical use. Blood atropine was determined by high performance liquid chromatography （HPLC）. Result The recovery rate of low, middle and high concentrations of blood atropine ranged 107.0% - 110.7%, with the relative standard deviation （RSD） of 0.95%-7.8%. Blood atropine was 74.51μg/ml before hemoperfusion. After perfusion for 1.0h, 2.0h and 3.0h, blood atropine was 16.00μg/ml, 3.85μg/ml and 1.51μg/ml, respectively, in the 0.5g group, decreased to 6.45μg/ml, 0.81μg/mi, 0.21μg/ml, respectively, in the 1.0g group, and was 4.50μg/ml, 0.43μg/ml, 0.32μg/ ml, respectively, in the 1.5g group. Conclusion Blood atropine can be quickly and accurately determined by HPLC. Hemoperfusion with membrane-coated activated charcoal can also remove atropine sulfate efficiently. Therefore, blood atropine must be monitored, and appropriate dose of atropine must be supplemented in time in clinical hemoperfusion.