Gemcitabine does not prevent acute rejection of the transplanted liver in rats |
| |
| Authors: | Hynek Mergental Jan Kriz Eva Honsova Michal Kudla Tomas Pantoflicek Katerina Tcherentsova Matej Kocik Frantisek Saudek Miroslav Ryska |
| |
| Institution: | Department of Transplant Surgery, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic;Centre of Diabetes, Institute for Clinical and Experimental Medicine, Prague, Czech Republic;Department of Pathology, Institute for Clinical and Experimental Medicine, Prague, Czech Republic |
| |
| Abstract: | Abstract Our study was designed to determine effect of gemcitabine on acute rejection of liver in rats. Liver transplantation was performed in rats of the Dark Agouti (DA) and Lewis (LEW) strains. Recipients were divided into three groups: A, DA-to-LEW without immunosuppression; B, DA-to-LEW, treated with cyclosporine A; C, DA-to-LEW, treated with gemcitabine. Immunosuppressants were subcutaneously injected for seven consecutive days after transplantation. On day 7, blood samples and liver graft tissue specimens were harvested. Group A showed severe rejection changes (RAI 8/9); in group B no rejection changes were present (RAI 0/9), and in group C moderate rejection changes were observed (RAI 6/9). Differences were significant between B vs C and A vs C groups; P >0.05. Serum creatinine and urea levels in the gemcitabine group were significantly lower than those in the cyclosporine A group. We did not confirm gemcitabine ability to prevent liver allograft rejection. |
| |
| Keywords: | Liver transplantation Acute rejection Immunosuppression Gemcitabine Rat |
|
|
