| HPLC法同时测定吴茱萸中吴茱萸苦素和柠檬苦素的含量 |
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| 引用本文： | 李丽,贾英,陈晓辉,毕开顺.HPLC法同时测定吴茱萸中吴茱萸苦素和柠檬苦素的含量[J].中国天然药物,2003,1(3):158-160. |
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| 作者姓名： | 李丽 贾英 陈晓辉 毕开顺 |
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| 作者单位： | 沈阳药科大学,沈阳,110016 |
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| 基金项目： | 国家“九五”科技攻关项目基金资助~~ |
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| 摘 要： | 目的 :建立吴茱萸中两种柠檬苦素类物质吴茱萸苦素和柠檬苦素含量的同时测定方法。方法 :采用反相高效液相色谱法 ,使用C18柱 ,乙腈 水 (36∶6 4 ,v/v)作为流动相 ,以 1 0ml/min流速洗脱 ,在 2 0 4nm波长下检测。结果 :吴茱萸中吴茱萸苦素和柠檬苦素分别在 4 0 4～ 4 0 4 μg·ml-1(r =0 9998,n =6 )和 3 96～ 39 6 μg·ml-1(r =0 9999,n =6 )范围内线性关系良好 ,加样回收率分别为 99 2 % (RSD 1 4 % )和 98 8% (RSD 1 2 % )。结论 :该方法准确 ,灵敏 ,便捷
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| 关 键 词： | HPLC法 测定 吴茱萸 吴茱萸苦素 柠檬苦素 含量 中药 |
| 文章编号： | 1000-5048(2003)03-0158-03 |
| 修稿时间： | 2003年6月25日 |
Determination of Rutaevine and Evodin in Evodia rutaecarpa by High-performance Liquid Chromatographic Method |
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| LI Li,JIA Ying,CHEN Xiao-Hui,BI Kai-Shun Shenyang Pharmaceutical University,Shenyang ,China.Determination of Rutaevine and Evodin in Evodia rutaecarpa by High-performance Liquid Chromatographic Method[J].Chinese JOurnal of Natural Medicines,2003,1(3):158-160. |
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| Authors: | LI Li JIA Ying CHEN Xiao-Hui BI Kai-Shun Shenyang Pharmaceutical University Shenyang China |
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| Institution: | LI Li,JIA Ying,CHEN Xiao-Hui,BI Kai-Shun * Shenyang Pharmaceutical University,Shenyang 110016,China |
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| Abstract: | AIM:To set up a quantitative high-performance liquid chromatographic method for the simultaneous determination of rutaevine and evodin in Evodia rutaecarpa. METHOD:An RP-HPLC method was set up, using Hypersil-C18 column, acetonitrile∶water (36∶64, v/v) as mobile phase with a flow rate of 1.0 ml/min at the detection wavelength of 204 nm. RESULT:The calibration curve was linear in the range of 4.04～40.4 μg*ml-1for rutaevine (r=0.9998, n=6) and 3.96～39.6 μg*ml-1 for evodin (r=0.9999, n=6) respectively. The average recoveries were 99.2% (RSD 1.4%) and 98.8% (RSD 1.2%) respectively. CONCLUSION:This method is accurate, sensitive and convenient. |
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| Keywords: | Wuzhuyu Rutaevine Evodin HPLC |
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