缺氧诱导因子-1α在胶原诱导性关节炎大鼠滑膜中的表达和意义

目的： 探讨缺氧诱导因子-1α(HIF-1α)在类风湿关节炎(RA)发病中的作用。方法： 采用雄性Wistar大鼠建立Ⅱ型胶原诱导性关节炎(CIA)模型，动态观察CIA大鼠关节炎活动指标，并于造模第21、28、35 d取膝关节滑膜分别行HE染色及HIF-1α免疫组化染色，分析HIF-1α蛋白表达与CIA大鼠关节炎活动指标、滑膜病理学评分之间的关系。结果： CIA大鼠膝关节滑膜衬里层和衬里下层细胞胞浆和胞核均表达HIF-1α，且其表达随发病时间延长而逐渐增加；滑膜表达HIF-1α与CIA大鼠关节炎活动指标、滑膜病理学总评分、滑膜增生评分及血管生成评分呈显著正相关，与炎症浸润评分无明显相关。结论： HIF-1α可能通过促进滑膜增生及血管生成而参与RA发病。

Expression and significance of hypoxia -inducible factor -1α on synovium from collagen- induced arthritis

AIM: To investigate the role of hypoxia-inducible factor-1α (HIF-1α) in the pathogenesis of rheumatoid arthritis (RA).METHODS: The collagen-induced arthritis (CIA) model was set up in male Wistar rats.The arthritis activity indexes,pathological changes and expression of HIF-1α on synovium at different time were observed through HE and immunohistochemistry staining.The correlations of HIF-1α expression with arthritis activity indexes and pathological scores were analyzed.RESULTS: Cytoplasmic and nucleic HIF-1α expressions were found on both lining and sublining area of synovium.HIF-1α expression in synovium increased gradually with the prolonged disease course.Synovial HIF-1α expression correlated significantly with arthritis activity indexes,total pathological score,synovial hyperplasia score and angiogenesis score,but not with inflammation score.CONCLUSION: HIF-1α may play important roles in the pathogenesis of RA through inducing synovial hyperplasia and angiogenesis.