自身免疫性糖尿病患者CD_4~+CD_(25)~+T细胞的变化

目的通过观察自身免疫性糖尿病患者外周血CD4+CD25+T细胞的变化,探讨其在发病中的可能作用。方法流式细胞仪检测20例LADA、20例T1DM、20例T2DM及21例正常对照外周血CD4+CD25+、CD3+CD8+T细胞。结果①LADA、T1DM组外周血CD4+CD25+T细胞低于T2DM、正常对照组(p均<0.05)。②LADA、T1DM患者外周血CD3+CD8+T细胞较T2DM、正常对照组增高(LADAvs正常对照p<0.01,其余p<0.05)。结论自身免疫性糖尿病患者免疫调节性CD4+CD25+T细胞减少,不能有效维持对胰岛自身抗原的耐受;外周细胞毒性CD3+CD8+T细胞增多进而破坏胰岛β细胞,导致自身免疫性糖尿病的发生和发展。

CHANGE OF CD+4 CD+25 T CELL SUBSET IN AUTOIMMUNE DIABETES PATIENTS

Objective To investigate the frequency and etiopathogenetic relevance of CD+4CD+25 immunoregulatory T cells in autoimmune diabetes mellitus (ADM). Methods Peripheral blood samples were obtained from 20 LADA,20 type 1 diabetes,20 type 2 diabetic patients and 21 controls. The frequency of peripheral CD+4CD+25 double-positive,CD+3CD+8 double-positive cells were measured by flow cytometry. Results ① We found lower numbers of CD+4CD+25 T cells in LADA and T1DM patients than in T2DM and control groups(p<0.05). ②Higher frequency of CD+3CD+8 T cells were found in LADA and T1DM group than in T2DM and control ones (LADA vs control p<0.01,others p<0.05). Conclusion The frequency of CD+4CD+25 immunoregulatory T cells decreases in ADM patients,which cannot maintain the tolerance to islet autoantigens effectively. Cytotoxic CD+3CD+8 T cells in the periphery simultaneously increase to attack and destroy islet β cells,leading to the development and progression of autoimmune diabetes.