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| 异鼠李素对Eca-109细胞DNA的合成和c-myc基因表达近日节律的影响 | |
| 引用本文： | 杨春蕾,彭涛,屈艺,陶大昌,王正荣,朱彬. 异鼠李素对Eca-109细胞DNA的合成和c-myc基因表达近日节律的影响[J]. 生物医学工程学杂志, 2005, 22(6): 1227-1230 |
| 作者姓名： | 杨春蕾 彭涛 屈艺 陶大昌 王正荣 朱彬 |
| 作者单位： | 1. 四川大学,生命科学院,医学细胞生物学教研室,成都,610041 2. 四川大学,基础医学与法医学院,生物医学工程教研室,成都,610041 3. 四川大学,基础医学与法医学院,生物化学与分子生物学教研室,成都,610041 |
| 基金项目： | 四川省科技厅基金资助项目(O4SG011-036) |
| 摘 要： | 为了探讨人食道癌E ca-109细胞DNA合成与c-m yc基因表达的近日节律以及异鼠李素对二者的影响作用,达到有效治疗肿瘤的目的。我们用3H-T dR掺入实验和流式细胞仪检测在24 h内不同时间点异鼠李素对E ca-109细胞DNA合成和c-m yc表达的近日节律的影响,其结果用方差分析和Cos inor法进行统计学分析。实验数据显示人食道癌E ca-109细胞株DNA合成和c-m yc基因的表达随近日节律变化,且异鼠李素对DNA合成和c-m yc基因的表达具有抑制作用,并影响其近日节律特征。人食道癌E ca-109细胞的生长与c-m yc基因表达在20:00至0:00达到高峰,且异鼠李素在此时间段作用较强,这为制定肿瘤时辰化疗方案提供了有价值的参考。 |
| 关 键 词： | 异鼠李素 肿瘤 近日节律 基因表达 DNA合成 |
| 收稿时间： | 2004-03-16 |
| 修稿时间： | 2004-03-162004-12-13 |
Effect of Isorhmnetin on Circadian Rhythms of DNA Synthesis and Expression of c-myc Gene in Eca-109 Cells of Human Oesophageal Cancer |
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| Yang Chunlei,Peng Tao,Qu Yi,Tao Dachang,Wang Zhengrong,Zhu Bin. Effect of Isorhmnetin on Circadian Rhythms of DNA Synthesis and Expression of c-myc Gene in Eca-109 Cells of Human Oesophageal Cancer[J]. Journal of biomedical engineering, 2005, 22(6): 1227-1230 | |
| Authors: | Yang Chunlei Peng Tao Qu Yi Tao Dachang Wang Zhengrong Zhu Bin |
| Affiliation: | 1.Department of Medical Cell Biology, Life Science College, Sichun University, Chengdu 610041,China; 2.Department of Biomedical Engineering, School of Basic Medical Sciences and Forensic Medicine, Sichun University,Chengdu 610041,Chiua; 3.Department of Medical Molecular Biology and Biochemistry, School of Basic Medical Sciences and Forensic Medicine,Sichuan University, Chengdu 610041,China |
| Abstract: | This study was focused on the circadian rhythms of DNA synthesis and the expression of c-myc gene in untreated and treated Eca-109 cells in human oesophageal cancer with isorhmnetin.The circadian rhythms of ~3H-TdR incorporation and expression of c-myc gene in untreated and treated Eca109 cells were measured by 3H-thymidine uptake assay and flow cytometry.The data collected were analyzed by ANOVA and Cosinor method.DNA synthesis and expression of c-myc gene in untreated group varied according to circadian time with statistical significance,the distribution curves of both DNA synthesis and the expression level of c-myc were fit for cosinor changes.The circadian rhythms of DNA synthesis and circadian parameters of c-myc expression in treated Eca-109 cells changed.The circadian parameters of DNA synthesis and expression level of c-myc varied after treatment by isorhmnetin.The effects of isorhmnetin on cell proliferation and c-myc expression reached the highest level from 20:00 to 0:00.The results provide a guidance for instituting the chemotherapy and chronotherapy of human tumors,when isorhmnetin is for use as anti-cancer agent. |
| Keywords: | Isorhmnetin Cancer Circadian rhythm Gene expression DNA synthesis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |