Cell shrinkage stimulates bradykinin-induced cell membrane potential oscillations in NIH 3T3 fibroblasts expressing the ras-oncogene |
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Authors: | M Ritter E Wöll S Waldegger D Häussinger H J Lang W Scholz B Schölkens F Lang |
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Institution: | (1) Physiologisches Institut der Universität Innsbruck, Fritz-Pregl-Strasse 3, A-6010 Innsbruck, Austria;(2) Medizinische Klinik der Universität Freiburg, Hugstetterstrasse 55, W-7800 Freiburg, Germany;(3) Hoechst A. G., W-6230 Frankfurt, Germany;(4) Physiologisches Institut der Universität Tübingen, Gmelinstrasse 5, W-7400 Tübingen, Germany |
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Abstract: | In NIH 3T3 fibroblasts expressing the Ha-ras oncogene (+ras) bradykinin leads to sustained oscillations of cell membrane potential due to oscillations of intracellular Ca2+ with subsequent activation of Ca2+-sensitive K+ channels. In cells not expressing the oncogene (-ras), bradykinin leads only to a single transient hyperpolarization of the cell membrane. The present study has been performed to elucidate the possible interaction of cell volume, intracellular pH and bradykinin-induced oscillations of the cell membrane potential. Bradykinin leads to cell shrinkage and intracellular alkalinization of both +ras cells and –ras cells. Inhibition of Na+/H+ exchanger by HOE 694 abolishes the bradykinin-induced alkalinization but does not significantly interfere with the bradykinin-induced oscillations of cell membrane potential. In contrast, prevention of bradykinin-induced cell shrinkage by simultaneous reduction of extracellular osmolarity blunts the oscillations. Thus, cell shrinkage stimulates bradykinin-induced oscillations of cell membrane potential. On the other hand, cell shrinkage alone does not elicit oscillations unless, in addition, Ca2+ entry is stimulated by ionomycin. |
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Keywords: | Cell volume Intracellular pH ras oncogene Calcium oscillations Cell membrane potential Bradykinin |
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