酸枣仁提取物对小鼠吗啡条件性位置偏爱的影响

目的:观察酸枣仁提取物对吗啡(MOR)诱导建立小鼠条件性位置偏爱(CPP)行为的影响。方法:连续给予吗啡(6mg·kg-1,sc)5d,引起小鼠产生显著的CPP效应,观察酸枣仁提取物对CPP效应的影响。结果:吗啡可诱导小鼠对伴药箱产生显著的位置偏爱;训练阶段于每次给予吗啡前30min给予酸枣仁醇提物800mg·kg-1可拮抗小鼠对吗啡产生的CPP效应(与吗啡对照组比较P<0.05),但酸枣仁醇提物200和400mg·kg-1不影响其效应(P>0.05);酸枣仁乙酸乙酯提取物13mg·kg-1、20mg·kg-1,正丁醇提取物160mg·kg-1可拮抗小鼠对吗啡产生的CPP效应(P<0.05),但酸枣仁石油醚提取物、乙醚提取物、水提物均不影响其效应(P>0.05)。结论:酸枣仁醇提物及其乙酸乙酯提取物、正丁醇提取物能在一定程度上抑制吗啡诱导小鼠建立的CPP行为。

EFFECTS OF ACTIVE FRACTION OF SEMEN ZIZIPHI SHINOSAE ON MORPHINE INDUCED CONDITIONED PLACE PREFERENCE IN MICE

Objective:To investigate the effects of the active fraction of Semen Ziziphi Spinosae(SZS) on morphine-induced conditioned place preference(CPP)in mice. Methods:Daily subcutaneous injection of morphine(6 mg·kg-1) was administered to mice for 5 days to induce CPP in mice,and daily treatment with a series of extracts of SZS (ip) was given 30 min prior to the administration of morphine to observe the effect of SZS on morphine-induced CPP. Results:Morphine(6 mg·kg-1,sc) induced mice to obtain a significant CPP and the CPP model was reliable and stable. By pretreating with alcoholic extract of SZS 800 mg·kg-1 (but not 200 mg·kg-1 or 400 mg·kg-1),ip,before morphine injection,the morphine-induced CPP was antagonized significantly(P<0.05). Then by pretreating with acetoacetate(13 mg·kg-1,20 mg·kg-1),and n-butanol(160 mg·kg-1)extract of SZS,the CPP was also inhibited (P<0.05),but the CPP was not affected by petroleum benzin,ethereal and aqueous extract of SZS. Conclusion:The effects of the active fraction of SZS can suppress the acquisition of morphine induced CPP in mice.