胰岛素样生长因子结合蛋白-2在U251细胞中对FAK磷酸化的促进作用

目的探讨外源性胰岛素样生长因子结合蛋白-2（insulin-like growth factor binding protein-2,IGFBP-2）在人胶质母细胞瘤细胞株U251细胞中,对黏着斑激酶（focal adhesion kinase,FAK）磷酸化的影响,从而提示IGFBP-2增强肿瘤细胞迁移、侵袭的机制。方法通过免疫荧光染色及蛋白印记试验检测IGFBP-2对FAK磷酸化的影响。结果在U251细胞中,500ng/mlIGFBP-2作用5分钟后,FAK磷酸化增强近一倍（P〈0.05）,作用30min后,FAK磷酸化增强近2倍（P〈0.01）。结论IGFBP-2可能通过FAK信号通路促进胶质瘤细胞迁移和侵袭。

Effects of exogenous insulin-like growth Factor binding protein-2 on activation of focal adhesion kinase signaling pathway in U251 human glioblastoma cells

Objective To investigate the effects of exogenous insulin-like growth factor binding protein-2 (IGFBP-2) on activation of focal adhesion kinase (FAK) signaling pathway in U251 human glioblastoma cells.Methods Western blotting analysis and immunofluorescence staining were used to examine the effects of IGFBP-2 on phosphorylation of FAK in U251 cells.Results Compared with the control, phosphorylated FAK in U251 cells increased by nearly one-fold after treated with 500 ng/ml IGFBP-2 for 5min (P<0.05), while in U251 cells treated with 500ng/ml IGFBP-2 for 30min, phosphorylated FAK increased by nearly two-fold (P<0.01).Conclusions IGFBP-2 may enhance migration and invasion of glioma cells through FAK pathway.