前列腺素E_2(PGE_2)对CCI_4诱导急性肝损害细胞保护作用的实验研究

PGE_2对CCI_4诱导的大鼠急性肝损害具有确切的细胞保护作用,能明显降低血清ASW、AST(-m)、ALT。减轻肝脏郁血水肿,降低肝细胞坏死、脂肪变性和炎症浸润等指标评分,并肯定了FGE_2的膜稳定效应,这可能是PGE_2对肝细胞保护效应的主要机制之一,还提出CCl_4对肝细胞膜性结构的损害呈均一性,FGE_2对肝细胞膜和线粒体都具有保护效应。

EXPERIMENTAL STUDY OF CYTOPROTECTIVE EFFECT OF PROSTAGLANDIN E_2 (PGE_2) ON CARBON TETRACHLORIDE (CCI_2)-INDUCED LIVER INJURY IN RAT

It was experimentally demonstrated that PGE_2 could partially protect hepatocytes from CCl_4-induced acute injury in rat. The findings evidenced that PGE_2 significantly decreases the elevated serum AST, AST-m, ALT, and LW/BW induced by CCl_4. The semiquantita- tive scores of the hepatocyte necosis, glycogen consumption, steatosis, vacuolarodegeneration, swollened liver cell, inflammatory infiltration in PGE_2 protecting group were remark- ably lower than those in CCl_4-treating group. The enzymological data was essentially cone- ordent with pathologic features. The changes of AST-m/AST and liver cell ultrastructure showed that PGE_2 might strengthen and stabilize the plasma membrane, which might be one of the main mechanisms of cytoprotection by PGE_2 on liver damage. It was also suggested- that the destroying effect of CCl_4 on subcellular organs surrounded by plasmatic memb- rane might be identical, and that PGE_2 protected both plasma membrane and mitochond- ria to the same extent from CCl_4 hepatotoxicity.