Effects of fibroblast-keratinocyte interactions on the secretion of interleukin-8

Using two models for preparing skin equivalent, the effects of interactions between various types of fibroblasts and keratinocytes on their secretory activity in the production of interleukin-8 are studied. The secretion of an antiinflammatory cytokine is found to depend on the type and function of fibroblasts, the level of their proliferative activity, and the extent of formation of the dermal layer. Keratinocytes are capable of recognizing fibroblast function and stimulate or inhibit the secretion of interleukin-8 depending on the functional status. A new concept of hyperkeratosis and parakeratosis in psoriasis is proposed, which is based on a defect in the autoregulation of mediators, specifically, interleukin-8. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 121, N ^o 2, pp. 181–184, February, 1996 Presented by D. S. Sarkisov, Member of the Russian Academy of Medical Sciences