旋覆代赭汤对反流性食管炎模型大鼠食管黏膜组织及血浆NOS活力的影响

目的:观察旋覆代赭汤及原方甘补组方对反流性食管炎(RE)模型大鼠食管黏膜组织及血浆一氧化氮合成酶(NOS)活力的影响。方法:将110只雄性Wistar大鼠随机分成正常对照组、假手术组和造模组。对造模组采用"食管—十二指肠端侧吻合术"动物模型。术后5d将成活模型大鼠随机分为模型组、旋覆代赭汤原方组(原方组)、原方倍用甘补方组(甘补组)、西药对照组(西药组)。从术后第6天开始,正常对照组、假手术组、模型对照组给予生理盐水灌胃,药物治疗组分别给予旋覆代赭汤原方、原方倍用甘补方、西药枸橼酸莫沙必利灌胃,应用化学法观察食管组织及血浆NOS活力的表达情况。结果:模型组与正常组比较,NOS的活性亦显著提高(P0.01);原方组、甘补方组及西药组较模型组NOS的活性明显下降(P0.05);甘补组NOS活性降低更加显著,差异有统计学意义(P0.05)。结论:旋覆代赭汤能够通过影响食管组织及血浆NOS活力,改善食管组织的舒缩功能,且甘补组对于RE大鼠食管舒缩神经递质的影响优于旋覆代赭汤原方及西药枸橼酸莫沙必利。

The Effect of Xuanfu Daizhe Decoction on the Esophagus Mucosa and NOS Activity in Plasma of Rat Model of Reflux Esophagitis

Objective:To investigate the effect of Xuanfu Daizhe decoction and double the sweet-invigoration herb decoction on reflux esophagitis model of rat esophageal nitric oxide synthetase activity.Method:110 Male wistar rats were randomly divided into 3 groups.normal group , sham operation and model group. Given the model group the operation of esophagus through improved end-to-side anastomosis of the duodenum.Five days after the operation, randomly divided the alived rats into model control group, Xuanfu Daizhe decoction group,double the sweet-invigoration herb decoction group and western medicine control group. From the sixth day after the operation, gave NS normal saline to the normal group,sham operation group, and model control group, and gave the Xuanfu Daizhe decoction to the Xuanfu Daizhe decoction group, gave the double the sweet-invigoration herb decoction to double the sweet-invigoration herb decoction group, and gave Mosapride citrate to western medicine control group.Then observed the activities of nitricoxide synthetase. Results:The rats’ NOS activities of model group were significantly higher than normal group,the difference was statistically significant(P <0.01).Compare with the model group, the rats’ NOS activities of esophageal organization in Xuanfu Daizhe decoction group and double the sweet-invigoration herb decoction group and western medicine control group were significantly lower than model group, the difference was statistically significant(P <0.05).Double the sweet-invigoration herb decoction group were reduced more remarkable than Xuanfu Daizhe decoction and Western medicine group, with significant difference meaning (P <0.05).Conclusion:Xuanfu Daizhe decoction can improve the activity of esophageal organizations by effecting nitric oxide synthetase. And the double the sweet-invigoration herb decoction were more benfit than Xuanfu Daizhe decoction and Mosapride citrate.