白介素-6调节颗粒细胞雌孕激素分泌的作用

目的 :探讨白介素 -6 (IL -6 )对颗粒细胞分泌雌孕激素的影响。方法 :收集行 IVF-ET穿卵时的颗粒细胞作体外培养 ,在有或无 FSH条件下 ,以不同浓度的 rh IL-6作用于颗粒细胞 ,2 4、48、72和 96 h后收集培养液作雌二醇、孕酮测定 ,观察基因重组人白介素 -6 (rh IL-6 )对体外培养中的颗粒细胞分泌雌二醇、孕酮的影响 ;通过 m RNA狭缝杂交 (m RNA slotblot)检测颗粒细胞是否存在 IL-6 R m RNA。结果 :在无 FSH条件下 ,rh IL-6对颗粒细胞分泌雌二醇有抑制作用 ,对孕酮分泌的影响不明显 ;而加入 FSH后 ,发现 rh IL-6对 FSH刺激颗粒细胞所分泌的雌二醇、孕酮均有明显抑制作用。这些作用呈现一定的时间和剂量依赖性。结论 :rh IL-6可抑制 FSH刺激颗粒细胞所致的甾体激素分泌 ,从而参与调节卵巢功能。

Effects of Interleukin-6 on Basal and FSH-stimulated Secretion of Estrodiol and Progesterone by Human Granulosa Cells in vitro

Objective:To determine the effects of interleukin 6(IL 6) on secretion of estradiol and progesterone by human granulosa cells in vitro. Methods:Granulosa cells were obtained from infertile patients undergoing IVF ET treatment and cultured with serum free supplemented HAM's F 10 medium. In the absence or presence of FSH, granulosa cells were treated with different concentrations of rhIL 6. The media were collected in 24, 48, 72,and 96 h and assayed for estradiol and progesterone. IL 6R mRNA was determined by the way of RNA slot blot. Results:IL 6 had a significant inhibiting effect on estradiol secretion, especially in the presence of FSH. IL 6 inhibited FSH stimulated progesterone but not unstimulated progesterone release. The inhibition dependents on dosage and time. Conclusion:IL 6 had an inhibitory effects on steroidogenesis of granulosa cells by its special receptors, which may have regulatory effects on ovarian function.