肺癌荷瘤小鼠体内炎性微环境改变的研究

目的：探讨肺癌荷瘤小鼠体内炎性微环境中IL-6、IL-8、NF-KB和TNF-a的变化情况。方法：选用雄性C57小黑鼠24只，随机分成正常组和肿瘤组，每组12只。肿瘤组接种Lewis肺癌，正常组不做处理，待肿瘤直径达到0．5～1cm后，分别于第7天、第14天各组各处死6只C57小鼠，取其血清，ELISA法检测NF-KB、TNF-α、IL-6、IL-8。结果：肿瘤组的肿瘤相关炎性因子NF-KB、TNF-α、IL-6、IL-8明显高于正常组，差异具有显著统计学意义（P〈O．05）。结论；肺癌荷瘤小鼠体内促进肿瘤生长的炎性因子明显升高，炎性因子有望成为肺癌筛查及诊治效果评价的新指标。

Study on Inflammatory Microenvironment Change of Mice Bearing Lung Cancer

Objective：To investigate the changes of inflammatory microenvironment in which IL-6, IL-8, NF-KB and TNF-α for mice bearing lung cancer. Methods： 24 male C57 mice, were randomly divided into two groups：normal group and tumor group, 12 rats in each group. Tumor group was with Lewis lung cancer, normal group was not treated. When tumor diameter reach 0. 5-1l cm, we detected the serum NP-KB, TNF-α, IL-6 and IL-8 using ELISA method. Resuits：Tumor associated inflammatory factors NF-KB, TNF-α, IL-6, IL-8 in the tumor group was significantly higher than the normal group, the difference was statistically significant （P〈0. 05）. Conclusion： Inflammatory factors promote lung cancer growth significantly. Inflammatory factors are expected to become a new marker for lung cancer screening and diagnosis.