原因不明习惯性流产患者人类白细胞抗原-DQ区域基因多态性的研究

目的探讨原因不明习惯性流产易感性与人类白细胞抗原(HLA)-DQ区域基因多态性的关系.方法采用聚合酶链反应-限制性片段长度多态性方法(PCR-RFLP),分析32例原因不明习惯性流产患者(研究组)和54例无不良妊娠史正常妇女(对照组)的DQA1和DQB1基因型.结果研究组的DQB1*0604,0605等位基因频率(12.5%)较对照组频率(2.8%)显著增高(P＜0.05),DQB1*0501,0502等位基因频率(3.1%)较对照组频率(11.1%)显著降低(P＜0.05);两组间DQA1各等位基因频率无统计学差异.研究组的DQA1*01-DQB1*0604,0605单元型频率(12.5%)较对照组(2.8%)显著增高(P＜0.05).结论DQB1*0604,0605等位基因与DQA1*01-DQB1*0604,0605单元型可能是该病的易感基因与易感单元型,而DQB1*0501,0502等位基因可能是防止该病发生的保护性因子.

The study on human leucocyte antigen DQ region genes Polymorphism in unexplained habitual abortiion Patients

Objective To investigate whether human leucocyte antigen DQ (HLA DQ)genes polymorphism are related to the susceptibility to unexplained habitual abortion Methods HLA DQA1 and HLA DQB1 gene typings are performed by polymerase chain reaction restrictive fragment length polymorphism(PCR RFLP) technique in 32 cases of unexplained habitual abortion and 54 women with normal pregnancy history Results In unexplained habitual abortion group,the frequency of DQB1*0604,0605 allele is increased significantly compared with that of controls(12 5% and 2 8%, P <0 05) and the frequency of DQB1*0501,0502 allele is decreased significantly (3 1% and 11 1%, P <0 05) The frequency of DQA1 alleles do not differ significantly between two groups Furthermore, the frequency of DQA1*01/DQB1*0604,0605 haplotype in patients is significantly increased than that of controls (12 5% and 2 8%, P <0 05) Conclusion The results suggest that DQB1*0604,0605 allele and DQA1*01/DQB1*0604,0605 haplotype may be a susceptible gene and haplotype of unexplained habitual abortion, while DQB1*0501,0502 allele may be a protective factor for habitual abortion